Department of Veterinary Microbiology, College of Veterinary and Animal Science, CSK HPKV, Palampur, Himachal Pradesh India ; National Veterinary Institute, Technical University of Denmark (DTU), Copenhagen, Denmark.
Indian J Microbiol. 2012 Jun;52(2):289-91. doi: 10.1007/s12088-011-0200-8. Epub 2011 Aug 19.
Mycobacterium bovis and Mycobacterium tuberculosis infect both animals and humans. The disease epidemiology by these agents differs in developed and developing countries due to the differences in the implementation of the prevention and control strategies. The present study describes the detection of M. bovis and M. tuberculosis from specimens of lungs and pulmonary lymph nodes of four cattle died in an organized herd of 183 cattle in the state of Himachal Pradesh, India, with inconclusive skin test results. Identification and distinction of these closely related mycobacterial species was done by PCR-RFLP targeting hsp65 gene followed by spacer oligonucleotide typing. Mixed infection of M. bovis and M. tuberculosis was detected in one cattle.
牛分枝杆菌和结核分枝杆菌均可感染动物和人类。由于在实施预防和控制策略方面的差异，这些病原体的疾病流行病学在发达国家和发展中国家有所不同。本研究描述了从印度喜马偕尔邦一个有组织的 183 头牛牛群中 4 头死于不明原因的牛的肺和肺淋巴结标本中检测到牛分枝杆菌和结核分枝杆菌的情况，这些牛的皮试结果不确定。通过针对 hsp65 基因的 PCR-RFLP 以及间隔寡核苷酸分型来进行鉴定和区分这些密切相关的分枝杆菌种。在一头牛中检测到牛分枝杆菌和结核分枝杆菌的混合感染。
