基于2A(HP)基因对来自伊朗的葡萄扇叶病毒系统发育不同分离株的分子特征分析
Molecular Characterization of Phylogenetically Distinct Isolates of Grapevine fanleaf virus from Iran Based on 2A(HP) Gene.
作者信息
Sokhandan-Bashir Nemat, Hooshmand Azam, Delpasand-Khabazi Afsaneh
机构信息
Department of Plant Protection, University of Tabriz, 51664 Tabriz, Iran.
出版信息
Indian J Virol. 2012 Jun;23(1):50-6. doi: 10.1007/s13337-012-0057-4. Epub 2012 Mar 4.
Abstract
Movement and coat protein genes from Grapevine fanleaf virus (GFLV) isolates have been characterized previously from Iran. In this study, an optimized reverse transcription polymerase chain reaction protocol was established to amplify RNA2 genomic segment corresponding to the hypothetical protein (2A(HP)). The sequence of 2A(HP) was compared with that of previously reported GFLV strains/isolates from other countries which showed 82-86% sequence identities. The 2A(HP) gene from Iran appeared to be standing distinct from other isolates of GFLV when genetic distance- or parsimony-based phylogeneitc analyses were carried out. The present study for the first time reports characterization of Iranian isolate of GFLV based on 2A(HP) gene.
摘要
此前已对来自伊朗的葡萄扇叶病毒（GFLV）分离株的运动蛋白基因和外壳蛋白基因进行了表征。在本研究中，建立了一种优化的逆转录聚合酶链反应方案，以扩增与假定蛋白（2A(HP)）相对应的RNA2基因组片段。将2A(HP)的序列与先前报道的来自其他国家的GFLV菌株/分离株的序列进行了比较，序列同一性为82%-86%。当进行基于遗传距离或简约法的系统发育分析时，来自伊朗的2A(HP)基因似乎与GFLV的其他分离株不同。本研究首次报道了基于2A(HP)基因的GFLV伊朗分离株的表征。
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