Wetzel Thierry, Fuchs M, Bobko M, Krczal G
Centrum Gruene Gentechnik, Staatliche Lehr- und Forschungsanstalt, Breitenweg 71, D-67435 Neustadt an der Weinstrasse, Germany.
Arch Virol. 2002 Aug;147(8):1643-53. doi: 10.1007/s00705-002-0828-0.
The RNA 2 of the nepovirus Arabis mosaic virus (ArMV) encodes a polyprotein from which protein 2A is released by proteolytic cleavage at the N-terminus. The 2A gene of 19 ArMV isolates from different geographical origin and 9 distinct natural hosts was amplified by RT/PCR and subsequently cloned and sequenced. These 19 isolates and those from databanks were classified into four groups based on the size of the protein 2A which ranged from 233 to 280 amino acids, and sequence identities. Sequence variability was mainly located in the N-terminus of the proteins, whereas the core region and the C-terminus were conserved.
南芥菜花叶病毒（ArMV）属于线虫传多面体病毒属，其RNA 2编码一种多聚蛋白，该多聚蛋白的N端经蛋白水解切割后释放出蛋白2A。通过RT/PCR扩增了来自不同地理区域的19个ArMV分离株以及9种不同天然宿主的2A基因，随后进行克隆和测序。根据蛋白2A大小（233至280个氨基酸）及序列同一性，将这19个分离株与数据库中的分离株分为四组。序列变异性主要位于蛋白质的N端，而核心区域和C端则保守。
