Kriz R, Lin L L, Sultzman L, Ellis C, Heldin C H, Pawson T, Knopf J
Genetics Institute, Cambridge, MA 02140.
Ciba Found Symp. 1990;150:112-23; discussion 124-7. doi: 10.1002/9780470513927.ch8.
Phospholipase C (PLC) is shown to comprise at least nine isoforms. These isoforms can be separated into three structurally related classes. Within a class the isozymes have similar enzymological properties. In the case of the PLC gamma class, both isoforms may be regulated by tyrosine phosphorylation. For PLC gamma 1 we show that the tyrosine phosphorylation sites are contained within the SH2/SH3 region or 'modulatory domain'. The overexpression of PLC gamma 1 in Rat-2 cells results in increased phosphatidylinositol breakdown in response to PDGF treatment, demonstrating that PLC gamma 1 mediates this response. We note that thrombin activates PLC gamma 1 in addition to other PLC isoforms.
磷脂酶C（PLC）已显示至少包含九种同工型。这些同工型可分为三个结构相关的类别。在一个类别中，同工酶具有相似的酶学性质。就PLCγ类别而言，两种同工型都可能受酪氨酸磷酸化调节。对于PLCγ1，我们发现酪氨酸磷酸化位点包含在SH2/SH3区域或“调节域”内。在大鼠2细胞中过表达PLCγ1会导致在血小板衍生生长因子（PDGF）处理后磷脂酰肌醇分解增加，这表明PLCγ1介导了这种反应。我们注意到，凝血酶除了激活其他PLC同工型外，还激活PLCγ1。
