Arimura A, Nagata M, Watanabe A, Nakamura K, Takeuchi M, Harada M
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Experientia. 1990 Jul 15;46(7):739-42. doi: 10.1007/BF01939952.
The role of mast cells in active and passive anaphylactic shock was examined using the WBB6F1 mouse, a genetically mast cell-deficient strain. Lethal anaphylactic shock occurred at high incidence rates in mice actively sensitized to bovine serum albumin (BSA). The reaction was specific to BSA since the shock could not be elicited by human or guinea pig serum albumin in these animals. Lethal shock could be prevented by CV-3988 but not by cyproheptadine, which suggests that the shock is mediated by PAF but not by histamine and serotonin. Similarly, lethal shock was provoked by homologous antigens in mice which had been passively sensitized with allogeneic anti-benzylpenicilloyl (BPO) IgG1 monoclonal antibody or with allogeneic or xenogeneic anti-BSA antiserum, but not in those sensitized with allogeneic anti-BPO IgE monoclonal antibody. These findings suggest that mast cells are not necessarily required for anaphylactic shock in the mouse.
利用WBB6F1小鼠（一种遗传性肥大细胞缺陷品系）研究了肥大细胞在主动和被动过敏性休克中的作用。对牛血清白蛋白（BSA）主动致敏的小鼠中，致死性过敏性休克的发生率很高。该反应对BSA具有特异性，因为在这些动物中，人或豚鼠血清白蛋白不会引发休克。CV - 3988可预防致死性休克，但赛庚啶不能，这表明休克是由血小板活化因子（PAF）介导的，而非组胺和5 - 羟色胺。同样，用同种异体抗苄青霉素酰基（BPO）IgG1单克隆抗体或同种异体或异种抗BSA抗血清被动致敏的小鼠，会被同源抗原诱发致死性休克，但用同种异体抗BPO IgE单克隆抗体致敏的小鼠则不会。这些发现表明，小鼠过敏性休克不一定需要肥大细胞参与。
