Vostalova Jitka, Rajnochova Svobodova Alena, Galandakova Adela, Sianska Jarmila, Dolezal Dalibor, Ulrichova Jitka
Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2013 Jun;157(2):137-45. doi: 10.5507/bp.2013.036. Epub 2013 Jun 3.
Solar light generates inflammatory responses in exposed skin. These effects are generally attributed to UVB light. However, skin is expose d to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning beds. We examined the effects of a single exposure to UVA and UVB wavebands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin.
Hairless mice were irradiated with either UVA (10 or 20 J/cm²) or UVB (200 or 800 mJ/cm²). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined by Western blot. Skin MPO activity was monitored spectrophotometrically.
UVB induced up-regulation of interleukin-1β (IL-1β) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA caused increase in levels of tumor necrosis factor-alpha (TNF-α) and IL-6 after 4 h and up-regulated IL-10 and interleukin-12 (IL-12) after 24 h. The increase in MPO activity from infiltrated leucocytes was observed only in UVB irradiated animals. iNOS was up-regulated 4 h after UVA and UVB treatment. No significant effect on COX-2 expression was detected.
UVA and UVB light affected several inflammatory markers. For individual waveband, changes in plasma parameters did not correlate with those in skin. Thus evaluation of plasma samples cannot simply be replaced by determination in skin specimens and vice versa.
太阳光可在暴露的皮肤中引发炎症反应。这些效应通常归因于中波紫外线（UVB）。然而，由于长波紫外线（UVA）是阳光以及日光浴床中使用的辐射的主要组成部分，皮肤会暴露于大量的UVA光子中。我们研究了单次暴露于UVA和UVB波段对皮肤和血浆中细胞因子水平、髓过氧化物酶（MPO）活性、诱导型一氧化氮合酶（iNOS）和环氧化酶2（COX-2）在皮肤中的表达的影响。
对无毛小鼠分别进行UVA（10或20 J/cm²）或UVB（200或800 mJ/cm²）照射。在4/24小时后评估其效果。使用生物芯片细胞因子检测法评估血浆细胞因子水平。通过蛋白质免疫印迹法测定皮肤中的细胞因子、iNOS和COX-2水平。用分光光度法监测皮肤MPO活性。
UVB主要在4小时后诱导白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）上调，白细胞介素-10（IL-10）降低。相比之下，UVA在4小时后导致肿瘤坏死因子-α（TNF-α）和IL-6水平升高，在24小时后使IL-10和白细胞介素-12（IL-12）上调。仅在接受UVB照射的动物中观察到浸润白细胞的MPO活性增加。UVA和UVB处理4小时后iNOS上调。未检测到对COX-2表达的显著影响。
UVA和UVB光影响多种炎症标志物。对于单个波段，血浆参数的变化与皮肤中的变化不相关。因此，血浆样本的评估不能简单地被皮肤样本的测定所取代，反之亦然。
