Novikova M S, Miterev G Iu, Morozova N G, Shakhbazian G P, Bulycheva T I
Gematol Transfuziol. 1990 Apr;35(4):23-6.
A method for early immunological prognosis of acute lymphoblastic leukemia (ALL) relapses has been presented. It is based on the dynamic phenotyping of lymphoid blood cells from the patients during remission by the indirect immunofluorescence test using a specially composed panel with monoclonal antibodies (MCA) to differentiation antigens of early lymphopoiesis stages. The panel composed for monitoring the population of leukemic cells during remission, contained 6 MCA selected from 40 MCA samples with the known specificity, basing on the principle of the highest reactivity with lymphoblasts from patients, and minimal positive reactions with lymphocytes from normal subjects. Blood mononuclear cells from 10 patients with ALL were subjected to immunophenotyping in the panel with MCA during remission, before each course of polychemotherapy. A retrospective analysis of the immunophenotyping data has shown that populations of lymphoid cells carrying antigens of early differentiation stages in amounts significantly exceeding the limits of the standard values (M +/- 3 delta) appear among blood lymphocytes, practically, in all the patients, 1-4 months before the relapse of ALL has been proved.
