两个新的辣根过氧化物酶编码基因的基因组DNA结构
Genomic DNA structure of two new horseradish-peroxidase-encoding genes.
作者信息
Fujiyama K, Takemura H, Shinmyo A, Okada H, Takano M
机构信息
Department of Fermentation Technology, Osaka University, Japan.
出版信息
Gene. 1990 May 14;89(2):163-9. doi: 10.1016/0378-1119(90)90002-9.
Genomic DNAs encoding the horseradish peroxidase (HRP) isozymes, prxC2 and prxC3, were cloned and sequenced. By comparing the sequences of the HRP isozyme-encoding genes, prxC1a and prxC1b and their cDNA [Fujiyama et al., Eur. J. Biochem. 173 (1988) 681-687], , it was concluded that prxC2 and prxC3 consisted of four exons and three introns as in the prxC1 gene family. The position of introns in coding regions were the same in all four prx genes. Genes prxC2 and prxC3 coded for 347 and 349 amino acid (aa) residues, respectively, including putative signal sequences at the N termini. In the flanking regions of both genes, putative promoters and polyadenylation signals were found. Nucleotide sequence homology in the coding region was 71% between prxC1a and prxC2, and 66% between prxC1a and prxC3. The aa sequence homologies in plant and microbial peroxidases were compared.
对编码辣根过氧化物酶（HRP）同工酶prxC2和prxC3的基因组DNA进行了克隆和测序。通过比较编码HRP同工酶的基因prxC1a和prxC1b及其cDNA的序列[藤山等人，《欧洲生物化学杂志》173（1988）681 - 687]，得出结论：prxC2和prxC3与prxC1基因家族一样，由四个外显子和三个内含子组成。所有四个prx基因编码区内含子的位置相同。基因prxC2和prxC3分别编码347和349个氨基酸（aa）残基，在N端包括推定的信号序列。在这两个基因的侧翼区域，发现了推定的启动子和聚腺苷酸化信号。prxC1a与prxC2编码区的核苷酸序列同源性为71%，prxC1a与prxC3编码区的核苷酸序列同源性为66%。比较了植物和微生物过氧化物酶的氨基酸序列同源性。
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