Department of Chemistry, University of Houston, Houston, Texas 77204, USA.
J Phys Chem B. 2013 Jun 27;117(25):7554-8. doi: 10.1021/jp403817b. Epub 2013 Jun 14.
The specific binding between the two DNA strands in a double helix is one of the most fundamental and influential molecular interactions in biochemistry. Using force-induced remnant magnetization spectroscopy (FIRMS), we obtained well-defined binding forces of DNA oligomers, with a narrow force distribution of 1.8 pN. The narrow force distribution allows for directly resolving two DNA duplexes with a single base-pair difference in the same sample. Therefore, binding force can serve as a discriminating parameter for probing different DNA interactions. Furthermore, we observed that the binding forces depend on the position of the mismatching base pair. Our results show that FIRMS is capable of high-precision mechanical measurements of biochemical processes involving multiple DNA interactions and has the potential for characterizing the binding strength of materials based on DNA origami.
双螺旋中两条 DNA 链之间的特异性结合是生物化学中最基本和最有影响力的分子相互作用之一。我们使用力诱导残磁光谱法（FIRMS）获得了明确的 DNA 寡聚物结合力，其力分布很窄，为 1.8 pN。这种窄的力分布允许在同一样品中直接分辨出两个只有单个碱基对差异的 DNA 双链体。因此，结合力可以作为探测不同 DNA 相互作用的区分参数。此外，我们观察到结合力取决于错配碱基对的位置。我们的结果表明，FIRMS 能够高精度地测量涉及多个 DNA 相互作用的生化过程，并有可能基于 DNA 折纸术来表征材料的结合强度。
