De Silva Lashan, Yao Li, Xu Shoujun
Department of Chemistry, University of Houston, Houston, Texas 77204, USA.
Chem Commun (Camb). 2014 Sep 25;50(74):10786-9. doi: 10.1039/c4cc03305a. Epub 2014 Jun 11.
The resolution of molecular bonds and subsequent selective control of their binding are of great significance in chemistry and biology. We have developed a method based on the use of acoustic radiation force to precisely dissociate noncovalent molecular bonds. The acoustic radiation force is produced by extremely low-power ultrasound waves and is mediated by magnetic particles. We successfully distinguished the binding of antibodies of different subclasses and the binding of DNA duplexes with a single-base-pair difference. In contrast to most ultrasound applications in chemistry, the sonication probe is noninvasive and requires a sample volume of only a few microliters. Our method is thus viable for noninvasive and accurate control of molecular bonds that are widely encountered in biochemistry.
分子键的解离以及随后对其结合的选择性控制在化学和生物学中具有重要意义。我们开发了一种基于声辐射力的方法，用于精确解离非共价分子键。声辐射力由极低功率的超声波产生，并由磁性颗粒介导。我们成功地区分了不同亚类抗体的结合以及相差一个碱基对的DNA双链体的结合。与化学中大多数超声应用不同，超声探头是非侵入性的，仅需要几微升的样品体积。因此，我们的方法对于生物化学中广泛存在的分子键的非侵入性和精确控制是可行的。
