Vanderkerken K, Bouwens L, Wisse E
Laboratory for Cell Biology and Histology, Free University of Brussels, Belgium.
Hepatology. 1990 Jul;12(1):70-5. doi: 10.1002/hep.1840120112.
Pit cells, or large granular lymphocytes, with natural tumoricidal activity are found in the sinusoids of normal rat liver. Hepatic large granular lymphocytes are heterogeneous and can be subdivided into two subsets. These subsets were compared with peripheral-blood large granular lymphocytes and were found to differ phenotypically and functionally. Phenotypical differences included lower expression of the asialo-GM1 marker of natural killer cells, lower cellular density and many more small cytoplasmic granules in hepatic large, granular lymphocytes. Low-density hepatic large, granular lymphocytes were five to eight times more cytotoxic than blood large, granular lymphocytes on a per-cell basis as measured against YAC-1 cells and colon carcinoma cells. In addition, hepatic large, granular lymphocytes were able to lyse P-815 target cells, which are resistant to blood natural killer cells. Large, granular lymphocytes isolated from the liver also contained a subset with intermediate phenotypical and functional characteristics, possibly representing a transitional form between blood and "liver-specific" large, granular lymphocytes. The liver thus contains a specific population of highly activated or further differentiated large granular lymphocytes.
在正常大鼠肝脏的血窦中可发现具有天然杀肿瘤活性的肝实质细胞，即大颗粒淋巴细胞。肝大颗粒淋巴细胞具有异质性，可分为两个亚群。将这些亚群与外周血大颗粒淋巴细胞进行比较，发现它们在表型和功能上存在差异。表型差异包括自然杀伤细胞去唾液酸GM1标志物的表达较低、细胞密度较低以及肝大颗粒淋巴细胞中存在更多小的胞质颗粒。以YAC-1细胞和结肠癌细胞为靶细胞进行检测时，低密度肝大颗粒淋巴细胞的细胞毒性在单细胞基础上比血中大颗粒淋巴细胞高5至8倍。此外，肝大颗粒淋巴细胞能够裂解对血中自然杀伤细胞有抗性的P-815靶细胞。从肝脏分离出的大颗粒淋巴细胞还包含一个具有中间表型和功能特征的亚群，可能代表血中大颗粒淋巴细胞和“肝脏特异性”大颗粒淋巴细胞之间的过渡形式。因此，肝脏中含有一群特定的、高度活化或进一步分化的大颗粒淋巴细胞。
