Institute of Genetics and Animal Breeding, Jastrzębiec, Poland.
PLoS One. 2013 May 30;8(5):e64022. doi: 10.1371/journal.pone.0064022. Print 2013.
The aim of the study was to establish an optimized protocol of iron dextran administration to pig neonates, which better meets the iron demand for erythropoiesis. Here, we monitored development of red blood cell indices, plasma iron parameters during a 28-day period after birth (till the weaning), following intramuscular administration of different concentrations of iron dextran to suckling piglets. To better assess the iron status we developed a novel mass spectrometry assay to quantify pig plasma levels of the iron-regulatory peptide hormone hepcidin-25. This hormone is predominantly secreted by the liver and acts as a negative regulator of iron absorption and reutilization. The routinely used protocol with high amount of iron resulted in the recovery of piglets from iron deficiency but also in strongly elevated plasma hepcidin-25 levels. A similar protocol with reduced amounts of iron improved hematological status of piglets to the same level while plasma hepcidin-25 levels remained low. These data show that plasma hepcidin-25 levels can guide optimal dosing of iron treatment and pave the way for mixed supplementation of piglets starting with intramuscular injection of iron dextran followed by dietary supplementation, which could be efficient under condition of very low plasma hepcidin-25 level.
本研究旨在建立一种优化的葡聚糖铁给药方案，以更好地满足仔猪红细胞生成的铁需求。在此，我们监测了新生仔猪在出生后 28 天（直至断奶）内不同浓度葡聚糖铁肌内给药后红细胞指数和血浆铁参数的变化。为了更好地评估铁状态，我们开发了一种新的质谱分析方法来定量测定猪血浆中铁调节肽激素铁调素-25 的水平。这种激素主要由肝脏分泌，作为铁吸收和再利用的负调节剂。常规使用高剂量铁的方案虽然使仔猪从缺铁中恢复过来，但也导致血浆铁调素-25 水平显著升高。而使用低剂量铁的类似方案将仔猪的血液学状态改善到相同水平，同时保持低血浆铁调素-25 水平。这些数据表明，血浆铁调素-25 水平可以指导铁治疗的最佳剂量，并为仔猪的混合补充铺平道路，从肌内注射葡聚糖铁开始，然后进行饮食补充，在血浆铁调素-25 水平非常低的情况下可能是有效的。
