School of Chemistry & Chemical Engineering, Harbin Normal University, Shi Da Nan Road, Harbin 150025, China.
J Anal Methods Chem. 2013;2013:318961. doi: 10.1155/2013/318961. Epub 2013 May 14.
To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established. The chromatographic separation of the absorbed compounds and metabolites was achieved with an ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) under a gradient elution. The mobile phase was composed of acetonitrile and water buffered with ammonium acetate (10 mM) and formic acid (0.1%, V/V). Twelve absorbed compounds and four metabolites were found. Seven of the absorbed compounds were identified by ESI-MS. The identification of absorbed compounds might be helpful for the better understanding of the mechanisms underlying the pharmacological effects of WJSH capsule.
为了鉴定口服乌鸡圣化（WJSH）胶囊后大鼠血清中吸收的化合物，建立了一种超高效液相色谱-电喷雾电离质谱（UPLC/ESI-MS）方法。采用 ACQUITY UPLC BEH C18 柱（2.1mm×50mm，1.7μm）在梯度洗脱下实现了吸收化合物和代谢物的色谱分离。流动相由乙腈和水组成，用乙酸铵（10mM）和甲酸（0.1%，V/V）缓冲。发现了 12 种吸收化合物和 4 种代谢物。通过 ESI-MS 鉴定了其中 7 种吸收化合物。吸收化合物的鉴定可能有助于更好地理解 WJSH 胶囊药理作用的机制。
