Liu Haiyang, Ma Wei, Liu Q I, Wang Fashan, Cao Ling, Li Tienan, Zhang Ning, Geng Fang
College of Jiamusi, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.
Duoduo Pharmaceutical Co. Ltd., Jiamusi, Heilongjiang 154002, P.R. China.
Exp Ther Med. 2015 Aug;10(2):635-640. doi: 10.3892/etm.2015.2548. Epub 2015 Jun 5.
The Wujia Shenghua capsule (WSC) is derived from Sheng-Hua-Tang, a well-known traditional Chinese medicine compound prescription that has been widely applied during the postpartum period in Chinese communities for a number of years. The aim of the present study was to investigate the effect of WSC on uterine bleeding following medically-induced incomplete abortion in rats during early pregnancy. Incomplete abortions were induced in Wistar rats during early pregnancy using mifepristone combined with misoprostol. The effects of WSC treatment were investigated in terms of the duration and volume of uterine bleeding, the uterine index and shape, and various hemodynamic indexes. In addition, blood samples were collected to measure the levels of estradiol (E), fibronectin (FN) and laminin (LM) via a radioimmunoassay or enzyme-linked immunosorbent assay, while the expression levels of FN, estrogen receptor (ER) and progesterone receptor (PR) in the uterine tissues were determined by immunohistochemistry. The results demonstrated that WSC treatment markedly shortened the duration and reduced the volume of uterine bleeding when compared with the model group. Furthermore, WSC treatment significantly elevated the E level in the serum and the expression of the ER and PR in the uterine tissues, while notably decreasing the FN and LM levels in the serum and uterine tissues. In addition, the hemodynamic indexes were shown to improve with WSC treatment. These results demonstrated that WSC exerted an inhibitory effect on the bleeding caused by medical abortion, possibly through modulating the E, ER, PR, FN and LM levels.
五加生化胶囊（WSC）源自生化汤，生化汤是一种著名的中药复方制剂，多年来在中国社区产后广泛应用。本研究的目的是探讨WSC对妊娠早期药物流产不全致大鼠子宫出血的影响。在妊娠早期，使用米非司酮联合米索前列醇诱导Wistar大鼠流产不全。从子宫出血的持续时间和出血量、子宫指数和形态以及各种血流动力学指标方面研究WSC治疗的效果。此外，采集血样，通过放射免疫分析或酶联免疫吸附测定法测量雌二醇（E）、纤连蛋白（FN）和层粘连蛋白（LM）的水平，同时通过免疫组织化学法测定子宫组织中FN、雌激素受体（ER）和孕激素受体（PR） 的表达水平。结果表明，与模型组相比，WSC治疗显著缩短了子宫出血的持续时间并减少了出血量。此外，WSC治疗显著提高了血清中E水平以及子宫组织中ER和PR的表达，同时显著降低了血清和子宫组织中FN和LM的水平。此外，WSC治疗可改善血流动力学指标。这些结果表明，WSC可能通过调节E、ER、PR、FN和LM水平对药物流产引起的出血发挥抑制作用。
