Department of Public Health, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
Biomed Res Int. 2013;2013:352370. doi: 10.1155/2013/352370. Epub 2013 May 2.
Cardiac tissue regeneration is guided by stem cells and their microenvironment. It has been recently described that both cardiac stem/primitive cells and extracellular matrix (ECM) change in pathological conditions. This study describes the method for the production of ECM typical of adult human heart in the normal and pathological conditions (ischemic heart disease) and highlights the potential use of cardiac fibroblast-derived ECM for in vitro studies of the interactions between ECM components and cardiac primitive cells responsible for tissue regeneration. Fibroblasts isolated from adult human normal and pathological heart with ischemic cardiomyopathy were cultured to obtain extracellular matrix (biomatrix), composed of typical extracellular matrix proteins, such as collagen and fibronectin, and matricellular proteins, laminin, and tenascin. After decellularization, this substrate was used to assess biological properties of cardiac primitive cells: proliferation and migration were stimulated by biomatrix from normal heart, while both types of biomatrix protected cardiac primitive cells from apoptosis. Our model can be used for studies of cell-matrix interactions and help to determine the biochemical cues that regulate cardiac primitive cell biological properties and guide cardiac tissue regeneration.
心脏组织再生受干细胞及其微环境的调控。最近有研究表明，在病理条件下，心脏干/原始细胞和细胞外基质（ECM）都会发生改变。本研究描述了在正常和病理条件（缺血性心脏病）下产生成人心脏典型 ECM 的方法，并强调了心脏成纤维细胞衍生的 ECM 在体外研究 ECM 成分与负责组织再生的心脏原始细胞之间相互作用的潜在用途。从成人正常和缺血性心肌病心脏中分离的成纤维细胞进行培养以获得细胞外基质（生物基质），该基质由典型的细胞外基质蛋白（如胶原蛋白和纤维连接蛋白）和基质细胞蛋白（层粘连蛋白和腱糖蛋白）组成。脱细胞后，该基质用于评估心脏原始细胞的生物学特性：正常心脏来源的生物基质可刺激原始细胞的增殖和迁移，而这两种类型的生物基质都能保护原始细胞免于凋亡。我们的模型可用于研究细胞-基质相互作用，并有助于确定调节心脏原始细胞生物学特性和指导心脏组织再生的生化线索。
