Kojima Y, Tojo M, Goldmann D A, Tosteson T D, Pier G B
Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.
J Infect Dis. 1990 Aug;162(2):435-41. doi: 10.1093/infdis/162.2.435.
A rabbit model of catheter-related bacteremia was developed to study immunity to the capsular polysaccharide/adhesin (PS/A) of coagulase-negative staphylococci. Catheters colonized by coagulase-negative staphylococci were inserted into the right jugular vein and attached to a subcutaneous osmotic pump, and blood cultures were obtained over 14 days. Nonimmune rabbits were bacteremic for 6-8 days after infection, hypoglycemic, and hyperlipidemic and had strong immune responses to teichoic acid but not to PS/A. PS/A immunization, but not teichoic acid immunization, reduced the number of bacteremic days by approximately 60%, diminished the hypoglycemia and hyperlipidemia, and ablated the immune responses to teichoic acid. Passive infusion of PS/A-specific polyclonal and monoclonal antibodies using a separate, noninfected catheter-pump combination implanted in the left jugular protected against both bacteremia and hematogenous colonization of this contralateral catheter.
建立了导管相关菌血症的兔模型，以研究对凝固酶阴性葡萄球菌荚膜多糖/黏附素（PS/A）的免疫。将被凝固酶阴性葡萄球菌定植的导管插入右颈静脉并连接到皮下渗透泵，在14天内进行血培养。未免疫的兔子在感染后菌血症持续6 - 8天，出现低血糖和高脂血症，对磷壁酸有强烈免疫反应，但对PS/A无免疫反应。PS/A免疫而非磷壁酸免疫可使菌血症天数减少约60%，减轻低血糖和高脂血症，并消除对磷壁酸的免疫反应。使用植入左颈静脉的单独的、未感染的导管 - 泵组合被动输注PS/A特异性多克隆和单克隆抗体，可预防对侧导管的菌血症和血行定植。
