Lauxen I S, Oliveira M G, Rados P V, Lingen M W, Nör J E, Sant'ana Filho M
Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Oral Dis. 2014 Apr;20(3):e76-80. doi: 10.1111/odi.12136. Epub 2013 Jun 6.
Cancer is a multifactorial disease composed of cells that show somatic mutations and epigenetic changes. The aim of this study was to investigate the expression of proteins involved in the development and maintenance of epithelia, cell cycle regulation, and apoptosis in human oral squamous cell carcinoma (OSCC) tissue samples.
A tissue microarray containing 65 primary human OSCC specimens was immunolabeled for bcl-2, survivin, epidermal growth factor receptor (EGFR), p21, p53, p63, and cleaved caspase-3.
Samples were scored for percentage of positively stained tumor cells and staining intensity. A total immunostaining score was also calculated, using the product of percentage and intensity scores. All specimens showed high scores, > 75%, for p63 and survivin, and 75.4% of the specimens also presented high EGFR expression. All cases showed p53-positive cells. p21 showed a diffuse staining pattern. The percentage of cells positive for cleaved caspase-3 and bcl-2 was low.
The high frequency of tumor cells expressing p63 and survivin highlights the role of these proteins in the malignant transformation of oral epithelium. Collectively, our results suggest that p63 and survivin may constitute attractive targets for cancer therapy in patients with OSCC.
癌症是一种多因素疾病，由显示体细胞突变和表观遗传变化的细胞组成。本研究的目的是调查参与上皮细胞发育和维持、细胞周期调控以及细胞凋亡的蛋白质在人类口腔鳞状细胞癌（OSCC）组织样本中的表达情况。
对包含65例原发性人类OSCC标本的组织芯片进行免疫标记，检测bcl-2、生存素、表皮生长因子受体（EGFR）、p21、p53、p63和裂解的半胱天冬酶-3。
对样本的阳性染色肿瘤细胞百分比和染色强度进行评分。还通过百分比和强度评分的乘积计算总免疫染色评分。所有标本中p63和生存素的评分均较高，>75%，75.4%的标本EGFR表达也较高。所有病例均显示p53阳性细胞。p21呈弥漫性染色模式。裂解的半胱天冬酶-3和bcl-2阳性细胞的百分比很低。
表达p63和生存素的肿瘤细胞的高频率突出了这些蛋白质在口腔上皮恶性转化中的作用。总体而言，我们的结果表明，p63和生存素可能是OSCC患者癌症治疗的有吸引力的靶点。
