浅析 Survivin 在伴嚼烟的口腔鳞状细胞癌患者的血液学、生物化学和遗传学特征中的相关性。

An Insight into Survivin in Relevance to Hematological, Biochemical and Genetic Characteristics in Tobacco Chewers with Oral Squamous Cell Carcinoma.

机构信息

Department of Biochemistry, Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar 563103, Karnataka, India.

Allied Health and Basic Sciences, Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar 563103, Karnataka, India.

出版信息

Cells. 2023 May 22;12(10):1444. doi: 10.3390/cells12101444.

DOI:10.3390/cells12101444

PMID:37408277

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10217417/

Abstract

BACKGROUND

Survivin is an inhibitor of apoptosis protein (IAP), encoded by the Baculoviral IAP Repeat Containing 5 () gene located on q arm (25.3) on chromosome 17. It is expressed in various human cancers and involved in tumor resistance to radiation and chemotherapy. The genetic analysis of the gene and its protein survivin levels in buccal tissue related to oral squamous cell carcinoma (OSCC) in South Indian tobacco chewers has not been studied. Hence, the study was designed to quantify survivin in buccal tissue and its association with pretreatment hematological parameters and to analyze the gene sequence.

METHOD

In a single centric case control study, buccal tissue survivin levels were measured by ELISA. A total of 189 study subjects were categorized into Group 1 (n = 63) habitual tobacco chewers with OSCC, Group 2 (n = 63) habitual tobacco chewers without OSCC, and Group 3 (n = 63) healthy subjects as control. Retrospective hematological data were collected from Group 1 subjects and statistically analyzed. The gene was sequenced and data were analyzed using a bioinformatics tool.

RESULTS

Survivin protein mean ± SD in Group 1 was (1670.9 ± 796.21 pg/mL), in Group 2 it was (1096.02 ± 346.17 pg/mL), and in Group 3 it was (397.5 ± 96.1 pg/mL) with significance ( < 0.001). Survivin levels showed significance with cut-off levels of absolute monocyte count (AMC), neutrophil/lymphocyte ratio (NLR), and lymphocyte/monocyte ratio (LMR) at ( = 0.001). The unique variants found only in OSCC patients were T → G in the promoter region, G → C in exon 3, C → A, A → G, G → T, T → G, A → C, G → A in exon 4, C → A, G → T, G → C in the exon 5 region.

CONCLUSIONS

The tissue survivin level increased in OSCC patients compared to controls; pretreatment AMC, LMR, and NLR may serve as add-on markers along with survivin to measure the progression of OSCC. Unique mutations in the promoter and exons 3-5 were observed in sequence analysis and were associated with survivin concentrations.

摘要

背景

Survivin 是凋亡抑制蛋白（IAP）家族的一员，由位于染色体 17q 臂（25.3）上的 Baculoviral IAP Repeat Containing 5 () 基因编码。它在各种人类癌症中表达，并参与肿瘤对放疗和化疗的抵抗。在印度南部的嚼烟者中，尚未对口腔鳞状细胞癌（OSCC）相关的口腔颊黏膜组织中的基因及其蛋白 survivin 水平进行遗传分析。因此，本研究旨在通过 ELISA 定量检测颊黏膜组织中的 survivin，并分析其与预处理血液学参数的关系，同时分析基因序列。

方法

在一项单中心病例对照研究中，通过 ELISA 法检测 189 例研究对象颊黏膜组织中的 survivin 水平，将其分为三组：第 1 组（n=63）为有 OSCC 的习惯性嚼烟者，第 2 组（n=63）为无 OSCC 的习惯性嚼烟者，第 3 组（n=63）为健康对照组。回顾性收集第 1 组患者的血液学数据，并进行统计学分析。对基因进行测序，并使用生物信息学工具进行数据分析。

结果

第 1 组、第 2 组和第 3 组的 survivin 蛋白平均水平分别为（1670.9±796.21）pg/mL、（1096.02±346.17）pg/mL 和（397.5±96.1）pg/mL，差异有统计学意义（<0.001）。生存素水平与绝对单核细胞计数（AMC）、中性粒细胞/淋巴细胞比值（NLR）和淋巴细胞/单核细胞比值（LMR）的截断值具有显著相关性（=0.001）。在 OSCC 患者中仅发现的独特变异包括启动子区域的 T→G、外显子 3 中的 G→C、外显子 4 中的 C→A、A→G、G→T、T→G、A→C、G→A、外显子 5 中的 C→A、G→T、G→C。