School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University, 11-68, Koshien Kyuban-cho, Nishinomiya, Japan.
Anal Bioanal Chem. 2013 Aug;405(20):6555-61. doi: 10.1007/s00216-013-7088-6. Epub 2013 Jun 6.
A monodisperse molecularly imprinted polymer (MIP) for curcumin was first prepared by precipitation polymerization using methacrylamide (MAM) and 4-vinylpyridine as functional co-monomers, divinylbenzene as a crosslinker, and a mixture of acetonitrile and toluene as a porogen. The use of MAM as the co-monomer resulted in the formation of a monodisperse MIP and non-imprinted polymer (NIP). MIP and NIP, respectively, were monodispersed with a narrow particle size distribution (3.3 ± 0.09 and 3.5 ± 0.10 μm). In addition to shape recognition, hydrophobic and hydrogen-bonding interactions affected the retention and molecular-recognition of curcumin on the MIP. The MIP for curcumin could extract curcuminoids (curcumin, demethoxycurcumin, and bisdemethoxycurcumin) in Curcuma longa L.
首次通过沉淀聚合，以丙烯酰胺（MAM）和 4-乙烯基吡啶为功能共聚单体、二乙烯基苯为交联剂、乙腈和甲苯的混合物为致孔剂，制备了姜黄素的单分散分子印迹聚合物（MIP）。使用 MAM 作为共聚单体可形成单分散 MIP 和非印迹聚合物（NIP）。MIP 和 NIP 分别具有单分散性和较窄的粒径分布（3.3 ± 0.09μm 和 3.5 ± 0.10μm）。除形状识别外，疏水性和氢键相互作用还影响了姜黄素在 MIP 上的保留和分子识别。MIP 可提取姜黄根茎中的姜黄素类化合物（姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素）。
