Laboratório de Entomologia, Núcleo de Sistemática e Bioecologia de Insetos, Departamento de Biologia, Universidade Estadual da Paraíba, Campina Grande, PB, Brasil.
Rev Soc Bras Med Trop. 2013 Mar-Apr;46(2):178-84. doi: 10.1590/0037-8682-1727-2013.
The need for studies that describe the resistance patterns in populations of Aedes aegypti (Linnaeus) in function of their region of origin justified this research, which aimed to characterize the resistance to temephos and to obtain information on esterase activity in populations of Aedes aegypti collected in municipalities of the State of Paraíba.
Resistance to temephos was evaluated and characterized from the diagnostic dose of 0.352mg i.a./L and multiple concentrations that caused mortalities between 5% and 99%. Electrophoresis of isoenzymes was used to verify the patterns of esterase activity among populations of the vector.
All populations of Aedes aegypti were resistant to temephos, presenting a resistance rate (RR) greater than 20. The greatest lethal dose 50% of the sample (CL50) was found for the municipality of Lagoa Seca, approximately forty-one times the value of CL50 for the Rockefeller population. The populations characterized as resistant showed two to six regions of α and β-esterase, called EST-1 to EST-6, while the susceptible population was only seen in one region of activity.
Aedes aegypti is widely distributed and shows a high degree of resistance to temephos in all municipalities studied. In all cases, esterases are involved in the metabolism and, consequently, in the resistance to temephos.
由于需要描述起源地区埃及伊蚊（Linnaeus）种群的抗药性模式，因此进行了这项研究，旨在对硫磷的抗药性进行特征描述，并获取在 Paraíba 州收集的埃及伊蚊种群中酯酶活性的信息。
通过 0.352mg i.a./L 的诊断剂量和导致死亡率在 5%至 99%之间的多个浓度来评估和描述硫磷的抗药性。同工酶电泳用于验证该媒介种群的酯酶活性模式。
所有埃及伊蚊种群均对硫磷产生抗药性，抗药性率（RR）大于 20。在 Lagoa Seca 市发现的样本半数致死剂量 50%（CL50）最高，约为 Rockefeller 种群 CL50 值的四十一倍。被确定为具有抗药性的种群显示出两种到六种 α 和 β-酯酶区域，称为 EST-1 到 EST-6，而敏感种群仅在一个活性区域中可见。
埃及伊蚊分布广泛，在所研究的所有城市均显示出对硫磷的高度抗药性。在所有情况下，酯酶均参与代谢，从而参与对硫磷的抗性。
