Grumet R, Fang G W
Department of Horticulture, Michigan State University, East Lansing 48824.
J Gen Virol. 1990 Jul;71 ( Pt 7):1619-22. doi: 10.1099/0022-1317-71-7-1619.
The 3' half of the RNA of the cucurbit potyvirus zucchini yellow mosaic virus (ZYMV) was genetically cloned and the cDNA sequence of a portion of the putative RNA polymerase gene, the complete coat protein gene and the 3' untranslated region was determined. The coat protein and putative polymerase genes are both part of a continuous open reading frame as is the case for other potyviruses whose genomes are expressed as polyproteins. The Q/S protease cleavage site for the N terminus of the coat protein was deduced by alignment of the coat protein and polymerase genes with other potyviral sequences. The resulting protein has 279 amino acids and a calculated Mr of 31,214. The predicted amino acid sequence indicates a ZYMV-unique N-terminal region and potyvirus-characteristic central and C-terminal regions. These data also verify that ZYMV is distinct from the cucurbit potyvirus watermelon mosaic virus 2.
对葫芦科马铃薯Y病毒西葫芦黄花叶病毒（ZYMV）RNA的3'端进行了基因克隆，并测定了部分假定RNA聚合酶基因、完整外壳蛋白基因及3'非翻译区的cDNA序列。与其他基因组表达为多聚蛋白的马铃薯Y病毒一样，外壳蛋白基因和假定的聚合酶基因均属于一个连续的开放阅读框。通过将外壳蛋白基因和聚合酶基因与其他马铃薯Y病毒序列比对，推导了外壳蛋白N端的Q/S蛋白酶切割位点。所得蛋白含有279个氨基酸，计算的分子量为31214。预测的氨基酸序列显示ZYMV有一个独特的N端区域以及马铃薯Y病毒特征性的中央和C端区域。这些数据也证实ZYMV与葫芦科马铃薯Y病毒西瓜花叶病毒2不同。
