Bryan G T, Gardner R C, Forster R L
DSIR Plant Protection, Auckland, New Zealand.
Arch Virol. 1992;124(1-2):133-46. doi: 10.1007/BF01314631.
The sequence of the 3'-terminal 1492 nucleotides of the genome of a New Zealand isolate of clover yellow vein potyvirus (CYVV) has been determined. This sequence encodes a large open reading frame of 1314 nucleotides, the start of which was not identified, but which encodes a putative 272 amino acid coat protein. Downstream of the coat protein coding region is a 177 nucleotide untranslated sequence terminated by a polyadenylate tract. Comparison of the deduced CYVV-NZ coat protein amino acid sequence with two other strains of CYVV showed 86-93% similarity, suggesting CYVV-NZ should be regarded as a separate CYVV strain. CYVV-NZ shares with other CYVV strains a direct repeat of 14-16 nucleotides that is capable of forming a stem-loop structure. Examination of 35 strains of 15 other potyviruses showed a similar stem-loop structure conserved in all cases. A possible role in replication is hypothesized for the structure.
已确定了来自新西兰的三叶草黄脉马铃薯Y病毒（CYVV）分离株基因组3'末端1492个核苷酸的序列。该序列编码一个1314个核苷酸的大开放阅读框，其起始位置未确定，但编码一种推定的272个氨基酸的外壳蛋白。外壳蛋白编码区下游是一个177个核苷酸的非翻译序列，由一个聚腺苷酸序列终止。将推导的CYVV-NZ外壳蛋白氨基酸序列与另外两个CYVV菌株进行比较，相似度为86-93%，这表明CYVV-NZ应被视为一个单独的CYVV菌株。CYVV-NZ与其他CYVV菌株共享一个14-16个核苷酸的直接重复序列，该序列能够形成茎环结构。对其他15种马铃薯Y病毒的35个菌株进行检测，结果表明在所有情况下都存在类似的保守茎环结构。推测该结构在复制中可能发挥作用。
