Niblett C L, Zagula K R, Calvert L A, Kendall T L, Stark D M, Smith C E, Beachy R N, Lommel S A
Plant Pathology Department, University of Florida, Gainesville 32611.
J Gen Virol. 1991 Mar;72 ( Pt 3):499-504. doi: 10.1099/0022-1317-72-3-499.
The 3'-terminal region of wheat streak mosaic virus (WSMV) genomic RNA was cloned and a cDNA sequence of 1809 nucleotides upstream of the poly(A) tract was determined. The sequence contains a single open reading frame of 1662 nucleotides and a 3' untranslated region of 147 nucleotides. Translation products from WSMV RNA and WSMV cDNA transcripts were immunoprecipitated by WSMV capsid protein antiserum, indicating that the 3'-terminal region of WSMV RNA encodes the capsid protein. Five potential N-terminal capsid protein protease cleavage sites were identified, which would yield proteins ranging from 31.7K to 46.8K. Alignment of the deduced amino acid sequence of the WSMV capsid protein with those of other potyviruses showed significant, but limited, identity as compared to the alignment of two or more aphid-transmitted potyviruses. Although WSMV has characteristics distinct from potyviruses, because of its particle morphology, translation strategy apparently based on polyprotein processing, the ability to form cytoplasmic cylindrical inclusions and the degree of capsid protein homology with aphid-transmitted potyviruses, it should be considered a member of the potyvirus group.
克隆了小麦线条花叶病毒（WSMV）基因组RNA的3'末端区域，并确定了多聚（A）尾上游1809个核苷酸的cDNA序列。该序列包含一个1662个核苷酸的单一开放阅读框和一个147个核苷酸的3'非翻译区。WSMV RNA和WSMV cDNA转录本的翻译产物被WSMV衣壳蛋白抗血清免疫沉淀，表明WSMV RNA的3'末端区域编码衣壳蛋白。鉴定出五个潜在的N末端衣壳蛋白蛋白酶切割位点，这将产生分子量在31.7K至46.8K之间的蛋白质。与两种或更多种由蚜虫传播的马铃薯Y病毒科病毒的比对相比，WSMV衣壳蛋白推导的氨基酸序列与其他马铃薯Y病毒科病毒的比对显示出显著但有限的同源性。尽管WSMV具有与马铃薯Y病毒科病毒不同的特征，由于其粒子形态、显然基于多聚蛋白加工的翻译策略、形成细胞质圆柱形内含体的能力以及与由蚜虫传播的马铃薯Y病毒科病毒的衣壳蛋白同源程度，但它应被视为马铃薯Y病毒科的成员。
