Anreddy Rama Narsimha Reddy, Yellu Narsimha Reddy, Devarakonda Krishna R
Department of Pharmacology, Vaageswari College of Pharmacy, Karimnagar, Andhra Pradesh, India.
Methods Mol Biol. 2013;1028:205-19. doi: 10.1007/978-1-62703-475-3_13.
Nanotechnology involves the creation and manipulation of materials at nanoscale levels to create products that exhibit novel properties. Engineered nanomaterials either metals (like carbon and silver) or metal oxides (like zinc oxide, magnesium oxide, and titanium oxide) induce toxicity and oxidative stress by generating free radicals. Various in vitro and in vivo models are available to estimate the oxidative stress induced by the nanoparticles. In this chapter, we describe the methods for the estimation of oxidative stress markers like reactive oxygen species (ROS), DNA damage estimation, and lipid peroxidation products; total antioxidant capacity (TAC) was mentioned.
纳米技术涉及在纳米尺度上创造和操控材料，以制造具有新奇特性的产品。工程纳米材料，无论是金属（如碳和银）还是金属氧化物（如氧化锌、氧化镁和二氧化钛），都会通过产生自由基来诱导毒性和氧化应激。有各种体外和体内模型可用于评估纳米颗粒诱导的氧化应激。在本章中，我们描述了评估氧化应激标志物的方法，如活性氧（ROS）、DNA损伤评估和脂质过氧化产物；还提到了总抗氧化能力（TAC）。
