Muñoz Egea Mari Carmen, Ji Pan, Pruden Amy, Falkinham Iii Joseph O
Department of Clinical Microbiology, Fundación Jiménez Díaz Hospital, 28040 Madrid, Spain.
Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA 24061, USA.
Pathogens. 2017 Sep 14;6(3):42. doi: 10.3390/pathogens6030042.
Both spp. and spp. are opportunistic premise plumbing pathogens that are found on pipe surfaces in households. However, examination of data published in prior microbiological surveys indicates that spp. and spp. tend not to coexist in the same household plumbing biofilms. That evidence led us to test the hypothesis that spp. in biofilms could inhibit the adherence of . Measurements of adherence of cells to stainless steel coupons using both culture and PCR-based methods showed that the presence of spp. biofilms substantially reduced adherence and vice versa. That inhibition of adherence was not reduced by UV-irradiation, cyanide/azide exposure, or autoclaving of the spp. biofilms. Further, there was no evidence of the production of anti-mycobacterial compounds by biofilm-grown spp. cells. The results add to understanding of the role of microbial interactions in biofilms as a driving force in the proliferation or inhibition of opportunistic pathogens in premise plumbing, and provide a potential new avenue by which exposures may be reduced for at-risk individuals.
两种[具体菌种1]和[具体菌种2]都是机会性室内管道病原体，存在于家庭管道表面。然而，对先前微生物学调查中公布的数据进行检查表明，[具体菌种1]和[具体菌种2]往往不会在同一家庭管道生物膜中共存。这一证据促使我们检验生物膜中的[具体菌种1]能够抑制[具体菌种2]黏附的假设。使用培养法和基于聚合酶链反应的方法对[具体菌种2]细胞黏附到不锈钢试片上的情况进行测量，结果表明[具体菌种1]生物膜的存在显著降低了[具体菌种2]的黏附，反之亦然。[具体菌种1]生物膜经紫外线照射、氰化物/叠氮化物处理或高压灭菌后，对[具体菌种2]黏附的抑制作用并未减弱。此外，没有证据表明生物膜生长的[具体菌种1]细胞会产生抗分枝杆菌化合物。这些结果有助于理解生物膜中微生物相互作用作为室内管道中机会性病原体增殖或抑制驱动力的作用，并为降低高危个体的[具体菌种2]暴露提供了一条潜在的新途径。
