Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Curr Opin Chem Biol. 2013 Aug;17(4):644-50. doi: 10.1016/j.cbpa.2013.05.015. Epub 2013 Jun 3.
Protein labeling by using a protein tag and its specific fluorescent probe is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. Recently, fluorogenic probes for protein labeling were developed. When using these probes, a washing step is not required for the removal of free probes from the cells, thus, allowing rapid detection of proteins in living cells with high signal-to-noise ratio. Various chemical principles have been applied in the designing of probes to include a turn-on fluorescence switch that is activated by the protein labeling reaction. In this review, we describe about the design strategy of the probes and the advances in fluorogenic protein labeling systems.
利用蛋白质标签及其特定荧光探针对蛋白质进行标记，正日益成为活细胞内实时成像蛋白质的一种有用技术。最近，开发出了用于蛋白质标记的荧光探针。使用这些探针时，无需洗涤步骤即可去除细胞内的游离探针，从而可以在活细胞中快速检测到具有高信噪比的蛋白质。在设计探针时应用了各种化学原理，包括通过蛋白质标记反应激活的荧光开关。在这篇综述中，我们描述了探针的设计策略以及荧光蛋白质标记系统的进展。
