Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
Biosens Bioelectron. 2013 Nov 15;49:164-9. doi: 10.1016/j.bios.2013.05.007. Epub 2013 May 14.
In this paper, a label-free and reagentless microRNA sensor based on an interpenetrated network of carbon nanotubes and electroactive polymer is described. The nanostructured polymer film presents very well-defined electroactivity in neutral aqueous medium in the cathodic potential domain from the quinone group embedded in the polymer backbone. Addition of microRNA miR-141 target (prostate cancer biomarker) gives a "signal-on" response, i.e. a current increase due to enhancement of the polymer electroactivity. On the contrary, non-complementary miRNAs such as miR-103 and miR-29b-1 do not lead to any significant current change. A very low detection limit of ca. 8 fM is achieved with this sensor.
本文描述了一种基于碳纳米管和电活性聚合物互穿网络的无标记和无试剂 microRNA 传感器。在中性水介质中，纳米结构聚合物薄膜在聚合物主链中嵌入的醌基团的阴极电位范围内表现出非常明确的电活性。添加 microRNA miR-141 靶标（前列腺癌生物标志物）会产生“信号开启”响应，即由于聚合物电活性增强而导致的电流增加。相反，非互补的 microRNAs，如 miR-103 和 miR-29b-1，不会导致任何显著的电流变化。该传感器的检测限低至约 8 fM。
