College of Electronic Information, Wuhan University, Wuhan, 430079, China.
Bioelectrochemistry. 2011 Jun;81(2):59-64. doi: 10.1016/j.bioelechem.2011.01.005. Epub 2011 Mar 31.
A novel electrochemical immunosensor built on three dimensional carbon nanotube-conducting polymer (CNT-CP) network is reported for detection of Hepatitis B surface antigen (HBsAg) in human serum. The CNT-CP network is prepared by drop-drying of CNT solution on glassy carbon electrode, followed by electrochemical polymerization of poly (pyrrole propionic acid) (pPPA) film to crosslink and stabilize the CNTs, wherein the CNTs form the backbone of the network, and offer great specific surface areas for antibody attachment, and confer good conductivity for electrochemical detection, while the conducting film integrates the carbon nanotubes into a stable network due to its self-limiting growth behavior and provides abundant carboxyl groups for covalent immobilization of probe proteins. As a unique matrix, the CNT-CP network enables sensitive electrochemical detection of HBsAg biomarker by using alkaline phosphatase (ALP)-conjugated secondary antibodies under sandwich format coupling with the ALP substrate solution, p-aminophenyl phosphate (PAPP), reaching a detection limit of 0.01ng/mL with a dynamic range of 5 orders of magnitude.
一种基于三维碳纳米管-导电聚合物（CNT-CP）网络的新型电化学免疫传感器被报道用于检测人血清中的乙型肝炎表面抗原（HBsAg）。CNT-CP 网络是通过将 CNT 溶液滴在玻碳电极上制备的，然后通过电化学聚合聚（吡咯丙酸）（pPPA）薄膜交联和稳定 CNT，其中 CNT 形成网络的骨架，为抗体附着提供了巨大的比表面积，并为电化学检测提供了良好的导电性，而导电膜由于其自限性生长行为将 CNT 集成到稳定的网络中，并提供丰富的羧基用于探针蛋白的共价固定。作为一种独特的基质，CNT-CP 网络通过夹心模式与碱性磷酸酶（ALP）偶联的二级抗体结合，并与 ALP 底物溶液对氨基苯磷酸（PAPP）反应，实现了对 HBsAg 生物标志物的敏感电化学检测，检测限为 0.01ng/mL，动态范围为 5 个数量级。
