Uniformed Services University and United States Military Cancer Institute, Bethesda, Maryland 20814, USA.
Clin Cancer Res. 2013 Jul 15;19(14):3966-76. doi: 10.1158/1078-0432.CCR-13-0616. Epub 2013 Jun 6.
Pseudomyxoma peritonei is an understudied cancer in which an appendiceal neoplasm invades the peritoneum and forms tumor foci on abdominal organs. Previous studies have shown that bacteria reside within pseudomyxoma peritonei tumors and mucin. Thus, we sought to analyze the effect of antibiotics on bacterial density and β-catenin expression within pseudomyxoma peritonei samples.
The study included 48 patients: 19 with disseminated peritoneal adenomucinosis (DPAM) and 29 with peritoneal mucinous carcinomatosis (PMCA). Fourteen patients were given antibiotics (30 mg lansoprazole, 1 g amoxicillin, and 500 mg clarithromycin) twice a day for 14 days. One week after completion of therapy, surgery was conducted and specimens were harvested for pathology, bacterial culture, ISH, and immunohistochemistry.
ISH showed the presence of bacteria in 83% of the patient samples, with a higher Helicobacter pylori density observed in PMCA versus DPAM. PMCA patients treated with antibiotics had a significantly lower bacterial density and decreased β-catenin levels in the cytoplasm, the cell nuclei, and mucin-associated cells. Although not significant, similar trends were observed in DPAM patients. Cell membrane β-catenin was significantly increased in both DPAM and PMCA patients receiving antibiotics.
Bacteria play an important role in pseudomyxoma peritonei. Antibiotic treatment improved the histopathology of tissue, particularly in PMCA patients. In PMCA, antibiotics decreased bacterial density and were associated with a significant β-catenin decrease in the cytoplasm, cell nuclei, and mucin along with a small membrane increase. These results suggest that antibiotics offer potential protection against cell detachment, cellular invasion, and metastasis.
腹膜假黏液瘤是一种研究不足的癌症，阑尾肿瘤侵犯腹膜并在腹部器官上形成肿瘤灶。先前的研究表明，细菌存在于腹膜假黏液瘤肿瘤和黏液中。因此，我们试图分析抗生素对腹膜假黏液瘤样本中细菌密度和β-连环蛋白表达的影响。
该研究纳入了 48 名患者：19 名患有弥漫性腹膜黏液腺癌（DPAM），29 名患有腹膜黏液性癌（PMCA）。14 名患者每天接受两次抗生素治疗（30mg 兰索拉唑、1g 阿莫西林和 500mg 克拉霉素），共 14 天。治疗结束后一周进行手术，采集标本进行病理、细菌培养、ISH 和免疫组化检查。
ISH 显示 83%的患者样本中存在细菌，PMCA 中的幽门螺杆菌密度高于 DPAM。接受抗生素治疗的 PMCA 患者的细菌密度显著降低，细胞质、细胞核和黏蛋白相关细胞中的β-连环蛋白水平降低。虽然不显著，但 DPAM 患者也观察到类似的趋势。接受抗生素治疗的 DPAM 和 PMCA 患者的细胞膜β-连环蛋白显著增加。
细菌在腹膜假黏液瘤中起重要作用。抗生素治疗改善了组织的组织病理学，特别是在 PMCA 患者中。在 PMCA 中，抗生素降低了细菌密度，并与细胞质、细胞核和黏蛋白中的β-连环蛋白显著降低有关，同时细胞膜略有增加。这些结果表明抗生素提供了针对细胞脱落、细胞侵袭和转移的潜在保护作用。
