Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, Texas, USA.
Lab Chip. 2013 Aug 7;13(15):2879-82. doi: 10.1039/c3lc41343h.
We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.
我们制作了一种由纤毛微柱组成的微流控装置，形成了一种多孔硅纳米线-微柱结构。我们证明了该原型设备可以优先捕获类外泌体脂质囊泡，同时过滤掉蛋白质和细胞碎片。通过在 PBS 缓冲液中溶解多孔纳米线，可以完整地回收捕获的脂质囊泡。
