Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Apartado 1095, 41080, Seville, Spain,
Environ Sci Pollut Res Int. 2013 Dec;20(12):8839-47. doi: 10.1007/s11356-013-1833-4. Epub 2013 Jun 7.
A glasshouse study of the coastal shrub Limoniastrum monopetalum was carried out to evaluate its tolerance and capacity to accumulate copper. We investigate the effects of Cu from 0 to 60 mmol l(-1) on the growth, photosynthetic apparatus, and nutrient uptake of L. monopetalum, by measuring gas exchange, chlorophyll fluorescence parameters, photosynthetic pigments, and total copper, nitrogen, phosphorus, sulfur, calcium, and magnesium content in the plant tissues. Although L. monopetalum did not survive at 60 mmol l(-1) Cu, the species demonstrated a high tolerance to Cu-induced stress, since all plants survived external Cu concentrations of up to 35 mmol l(-1) and displayed similar growth in the Cu-enriched medium as in the control treatment of up to the external level of 15 mmol Cu l(-1) (1,000 mg Cu l(-1)). The reduced growth registered in plants exposed to 35 mmol Cu l(-1) can be attributed to reduced photosynthetic carbon assimilation associated with the adverse effect of the metal on the photochemical apparatus and a reduction in the absorption of essential nutrients. Copper tolerance was associated with the capacity of the plant to accumulate the metal in its roots and effectively prevent its translocation to photosynthetic tissues. L. monopetalum has the characteristics of a Cu-excluder plant and could be used in the revegetation of Cu-contaminated soils.
采用温室盆栽试验研究了滨海灌木单药獐牙菜对铜的耐性和积累能力。通过测定气体交换、叶绿素荧光参数、光合色素以及植物组织中总铜、氮、磷、硫、钙和镁含量，研究了 0 至 60mmol/L 的 Cu 对单药獐牙菜生长、光合器官和养分吸收的影响。尽管单药獐牙菜在 60mmol/L 的 Cu 下不能存活，但该物种对 Cu 诱导的胁迫具有很高的耐性，因为所有植物在高达 35mmol/L 的外部 Cu 浓度下存活，并在富 Cu 培养基中表现出与对照处理（高达 15mmol Cu/L（1000mg Cu/L））相似的生长。暴露于 35mmol Cu/L 的植物的生长减少归因于与金属对光化学器官的不利影响相关的光合碳同化减少，以及必需养分吸收减少。铜耐性与植物在根部积累金属的能力以及有效防止其向光合组织转运的能力有关。单药獐牙菜具有 Cu 排斥植物的特征，可用于 Cu 污染土壤的植被恢复。
