Pitt William G, Perez Krystian X, Tam Ngai Keung, Handly Erika, Chinn Joseph A, Liu Xiaojun Michael, Maziarz Edmond Peter
Chemical Engineering Department, Brigham Young University, Provo, Utah, 84602.
J Biomed Mater Res B Appl Biomater. 2013 Nov;101(8):1516-23. doi: 10.1002/jbm.b.32973. Epub 2013 Jun 7.
The introduction of silicone hydrogel (SiHy) contact lenses to the consumer marketplace necessitates study of the susceptibility of these lenses to spontaneous deposition by hydrophobic lipid components of ocular tears. The use of radioisotopes to measure lipid sorption on SiHy contact lenses gives precise and accurate results but requires institutional infrastructure and compels efficient lipid removal from the lens. This study compares three methods of quantitating phospholipid and cholesterol sorption on SiHy lenses using radiolabeled cholesterol and phosphatidylcholine that were sorbed on lenses from an artificial tear fluid. A triple extraction technique using n-propanol gives the most reliable results. Comparison of sorption on SiHy lenses shows that balafilcon A and senofilcon A lenses sorb similar amounts, while lotrafilcon B lenses sorb comparatively less.
向消费市场推出硅水凝胶（SiHy）隐形眼镜需要研究这些镜片对眼泪中疏水性脂质成分自发沉积的敏感性。使用放射性同位素测量脂质在SiHy隐形眼镜上的吸附可得到精确准确的结果，但需要机构基础设施，并且需要有效地从镜片上去除脂质。本研究比较了三种使用放射性标记的胆固醇和磷脂酰胆碱来定量SiHy镜片上磷脂和胆固醇吸附的方法，这些放射性标记物是从人工泪液吸附到镜片上的。使用正丙醇的三重萃取技术可得到最可靠的结果。对SiHy镜片吸附情况的比较表明，balafilcon A镜片和senofilcon A镜片吸附量相似，而lotrafilcon B镜片吸附量相对较少。
