Probing the dimerization interface of Leishmania infantum trypanothione reductase with site-directed mutagenesis and short peptides.

Binding at the interface: We tested the inhibitory activity of a set of peptide sequences derived from an α-helix of the dimeric trypanothione reductase from Leishmania infantum. Replacement of a glutamic acid residue with a lysine promoted monomer dissociation and enzyme inhibition.