单一的 PLP 依赖性酶 PctV 催化 3-脱氢莽草酸转化为 pactamycin 生物合成中的 3-氨基苯甲酸。
A single PLP-dependent enzyme PctV catalyzes the transformation of 3-dehydroshikimate into 3-aminobenzoate in the biosynthesis of pactamycin.
机构信息
Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551, Japan.
出版信息
Chembiochem. 2013 Jul 8;14(10):1198-203. doi: 10.1002/cbic.201300153. Epub 2013 Jun 6.
PMID:23744829
Abstract
Natural amino donation: A PLP-dependent aminotransferase PctV, encoded in the pactamycin biosynthetic gene cluster, was found to catalyze the formation of 3-aminobenzoate from 3-dehydroshikimate with L-glutamate as the amino donor. The PctV reaction comprises a transamination and two dehydration reactions. This is the first report of a simple 3-ABA synthase in nature.
摘要
天然氨基酸供体
pactamycin 生物合成基因簇中编码的依赖 PLP 的转氨酶 PctV 被发现能够催化 3-去氢莽草酸与 L-谷氨酸作为氨基供体生成 3-氨基苯甲酸。PctV 反应包括转氨和两个脱水反应。这是自然界中第一个简单的 3-ABA 合酶的报道。
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