Chen Yan-Gan, Song Yue-Lin, Wang Ying, Yuan Yun-Fei, Huang Xiao-Jun, Ye Wen-Cai, Wang Yi-Tao, Zhang Qing-Wen
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau 999078, Macao.
Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China.
J Pharm Biomed Anal. 2014 May;93:51-8. doi: 10.1016/j.jpba.2013.05.017. Epub 2013 May 20.
Puerariae Radix was a widely used herbal medicine. Pueraria lobata (PL) and Pueraria thomsonii (PT) were the two authorized sources of Puerariae Radix (gegen) in China. In this study, metabolic differentiations between these two species were investigated using NMR spectroscopy followed by principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). The content of puerarin in PL and PT was also determined using quantitative (1)H NMR spectroscopy. Thirteen isoflavones were tentatively identified based on 1D and 2D NMR spectroscopic data in two species. The (1)H NMR spectra of PL and PT were obviously different. PL and PT could also be markedly discriminated from (1)H NMR spectroscopic data by PCA and PLS-DA. For the crude drug resources, isoflavones, in which puerarin is the most important one, were regarded as the reasonable markers for the discrimination of the two species. The contents of puerarin and total isoflavones in PL were quantitated much higher than those in PT. Above all, (1)H NMR spectroscopy, which can provide comprehensive profiles of the metabolites and achieve convenient determinations of puerarin and total isoflavones in a single run, is an efficient means for evaluating the medicinal samples and achieving a better quality control of Puerariae Radix.
葛根是一种广泛应用的草药。野葛（PL）和甘葛藤（PT）是中国法定的葛根药材来源。在本研究中，采用核磁共振波谱法，随后进行主成分分析（PCA）和偏最小二乘判别分析（PLS - DA），研究了这两个物种之间的代谢差异。还使用定量氢核磁共振波谱法测定了PL和PT中葛根素的含量。基于两个物种的一维和二维核磁共振波谱数据，初步鉴定了13种异黄酮。PL和PT的氢核磁共振谱明显不同。通过PCA和PLS - DA，也可以从氢核磁共振波谱数据中显著区分PL和PT。对于药材资源，异黄酮（其中葛根素是最重要的一种）被视为区分这两个物种的合理标志物。PL中葛根素和总异黄酮的含量经定量测定远高于PT。最重要的是，氢核磁共振波谱法能够提供代谢物的全面图谱，并在一次运行中方便地测定葛根素和总异黄酮，是评估药用样品和实现更好的葛根质量控制的有效手段。
