Zhang Hongguang, Liu Shuhua, Li Huaizhi, Xue Fumin, Han Shuxin, Wang Liang, Cheng Yan, Wang Xiao
Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences) Jinan 250014 China
College of Chemistry and Chemical Engineering, Ocean University of China Tsingtao Shandong 266100 China.
RSC Adv. 2018 Jun 20;8(40):22652-22658. doi: 10.1039/c8ra02653j. eCollection 2018 Jun 19.
As an alternative to organic solvents, subcritical water was employed for the first time as an effective solvent for the extraction of isoflavones from . Optimum experimental conditions for the extraction of the four main isoflavones were established by single factor experiments, and the optimum experimental conditions for total isoflavone extraction were established further by response surface methodology. With an extraction time of 45 min and a liquid/solid ratio of 1 : 20, the extraction yields of puerarin, 3'-methoxypuerarin, and daidzin reached maxima at extraction temperatures of 120 °C, 140 °C and 200 °C, respectively. Moreover, puerarin, 3'-methoxypuerarin and daidzin were degraded and produced various byproducts due to hydrothermal reactions at higher temperatures. The maximum extraction yields of the total isoflavones were obtained by response surface methodology (extraction time of 45 min, solid/liquid ratio of 1 : 15 and extraction temperature of 120 °C). Compared to conventional solvents, subcritical water utilized less solvent and required a shorter extraction time.
作为有机溶剂的替代物，亚临界水首次被用作从……中提取异黄酮的有效溶剂。通过单因素实验确定了提取四种主要异黄酮的最佳实验条件，并通过响应面法进一步确定了总异黄酮提取的最佳实验条件。在提取时间为45分钟、液固比为1∶20的情况下，葛根素、3'-甲氧基葛根素和大豆苷的提取率分别在120℃、140℃和200℃的提取温度下达到最大值。此外，由于在较高温度下的水热反应，葛根素、3'-甲氧基葛根素和大豆苷会降解并产生各种副产物。通过响应面法获得了总异黄酮的最大提取率（提取时间45分钟、固液比1∶15和提取温度120℃）。与传统溶剂相比，亚临界水使用的溶剂量更少，提取时间更短。
