Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital, Ningbo University, Ningbo, 315040, China.
Department of Otorhinolaryngology- Head and Neck Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China.
J Clin Lab Anal. 2021 Feb;35(2):e23646. doi: 10.1002/jcla.23646. Epub 2020 Dec 15.
Tissue engineering has been a topic of extensive research in recent years and has been applied to the regeneration and restoration of many organs including the larynx. Currently, research investigating tissue engineering of the larynx is either ongoing or in the preclinical trial stage.
A literature search was performed on the Advanced search field of PubMed using the keywords: "(laryncheal tissue engineering) AND (cartilage regeneration OR scaffolds OR stem cells OR biomolecules)." After applying the selection criteria, 65 articles were included in the study.
The present review focuses on the rapidly expanding field of tissue-engineered larynx, which aims to provide stem cell-based scaffolds combined with biological active factors such as growth factors for larynx reconstruction and regeneration. The trend in recent studies is to use new techniques for scaffold construction, such as 3D printing, are developed. All of these strategies have been instrumental in guiding optimization of the tissue-engineered larynx, leading to a level of clinical induction beyond the in vivo animal experimental phase.
This review summarizes the current progress and outlines the necessary basic components of regenerative laryngeal medicine in preclinical fields. Finally, it considers the design of scaffolds, support of growth factors, and cell therapies toward potential clinical application.
组织工程学是近年来广泛研究的课题，已应用于包括喉在内的许多器官的再生和修复。目前，研究喉组织工程学的研究要么正在进行，要么处于临床前试验阶段。
在 PubMed 的高级搜索字段中使用关键词“（喉组织工程学）和（软骨再生或支架或干细胞或生物分子）”进行文献检索。应用选择标准后，有 65 篇文章纳入研究。
本综述重点介绍了快速发展的组织工程化喉领域，该领域旨在提供基于干细胞的支架，并结合生长因子等生物活性因子，用于喉重建和再生。最近的研究趋势是开发用于支架构建的新技术，例如 3D 打印。所有这些策略都有助于指导组织工程化喉的优化，使临床诱导水平超越体内动物实验阶段。
本综述总结了目前的进展，并概述了临床前领域再生喉医学的必要基本组成部分。最后，它考虑了支架的设计、生长因子的支持以及细胞疗法在潜在临床应用中的作用。
