一种四组分脂质混合物的相图：二硬脂酰磷脂酰胆碱/二油酰磷脂酰胆碱/1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱/胆固醇
Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.
作者信息
Konyakhina Tatyana M, Wu Jing, Mastroianni James D, Heberle Frederick A, Feigenson Gerald W
机构信息
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
出版信息
Biochim Biophys Acta. 2013 Sep;1828(9):2204-14. doi: 10.1016/j.bbamem.2013.05.020. Epub 2013 Jun 7.
Abstract
We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld+Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld+Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
摘要
我们报告了脂质双层混合物DSPC/DOPC/POPC/chol（二硬脂酰磷脂酰胆碱/二油酰磷脂酰胆碱/1-棕榈酰-2-油酰磷脂酰胆碱/胆固醇）的首个四组分相图。该相图具有宏观的Ld+Lo相域，清楚地表明，针对含有DOPC的三组分混合物确定的所有相界都能平滑地过渡到含有POPC的三组分混合物的相界，后者具有纳米级的Ld+Lo相域。我们的研究从两个已发表的三元相图出发，展示了如何通过研究几百个中间组成的样品将它们合并成一个四元相图。
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本文引用的文献
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Phys Rev E Stat Nonlin Soft Matter Phys. 2013 Feb;87(2):022708. doi: 10.1103/PhysRevE.87.022708. Epub 2013 Feb 13.
2
Toward a better raft model: modulated phases in the four-component bilayer, DSPC/DOPC/POPC/CHOL.向着更好的筏模型前进：四组分双层 DSPC/DOPC/POPC/CHOL 中的调制相。
Biophys J. 2013 Feb 19;104(4):853-62. doi: 10.1016/j.bpj.2013.01.003.
3
Bilayer thickness mismatch controls domain size in model membranes.双层厚度失配控制模型膜中的域大小。
J Am Chem Soc. 2013 May 8;135(18):6853-9. doi: 10.1021/ja3113615. Epub 2013 Feb 22.
4
Phase behavior and domain size in sphingomyelin-containing lipid bilayers.含鞘磷脂的脂质双层膜的相行为和结构域大小
Biochim Biophys Acta. 2013 Apr;1828(4):1302-13. doi: 10.1016/j.bbamem.2013.01.007. Epub 2013 Jan 18.
5
Membrane heterogeneity: manifestation of a curvature-induced microemulsion.膜的非均质性：曲率诱导微乳液的表现
Phys Rev E Stat Nonlin Soft Matter Phys. 2012 Mar;85(3 Pt 1):031902. doi: 10.1103/PhysRevE.85.031902. Epub 2012 Mar 2.
6
Control of a nanoscopic-to-macroscopic transition: modulated phases in four-component DSPC/DOPC/POPC/Chol giant unilamellar vesicles.控制纳米到宏观转变：四组分 DSPC/DOPC/POPC/Chol 巨大单层囊泡中的调制相。
Biophys J. 2011 Jul 20;101(2):L8-10. doi: 10.1016/j.bpj.2011.06.019.
7
Phase separation in lipid membranes.脂膜中的相分离。
Cold Spring Harb Perspect Biol. 2011 Apr 1;3(4):a004630. doi: 10.1101/cshperspect.a004630.
8
Comparison of three ternary lipid bilayer mixtures: FRET and ESR reveal nanodomains.三种三元脂质双层混合物的比较：FRET 和 ESR 揭示纳米区。
Biophys J. 2010 Nov 17;99(10):3309-18. doi: 10.1016/j.bpj.2010.09.064.
9
Direct mapping of nanoscale compositional connectivity on intact cell membranes.在完整细胞膜上直接映射纳米级组成连通性。
Proc Natl Acad Sci U S A. 2010 Aug 31;107(35):15437-42. doi: 10.1073/pnas.1003876107. Epub 2010 Aug 16.
10
Fast molecular tracking maps nanoscale dynamics of plasma membrane lipids.快速分子追踪绘制出了质膜脂质的纳米尺度动力学图谱。
Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6829-34. doi: 10.1073/pnas.0912894107. Epub 2010 Mar 29.
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