基于DNA的药物递送纳米载体与滚环扩增的自组装。
Self-assembly of DNA-based drug delivery nanocarriers with rolling circle amplification.
作者信息
Ouyang Xiangyuan, Li Jiang, Liu Huajie, Zhao Bin, Yan Juan, He Dannong, Fan Chunhai, Chao Jie
机构信息
Division of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
National Engineering Research Center for Nanotechnology (NERCN), Shanghai 200241, China.
出版信息
Methods. 2014 May 15;67(2):198-204. doi: 10.1016/j.ymeth.2013.05.024. Epub 2013 Jun 7.
DNA nanostructures have recently emerged as a type of drug delivery nanocarriers due to their suitable sizes, well-defined structures and low-toxicity. Here, we present a protocol for the assembly of DNA nanoribbon structures with rolling circle amplification (RCA) and delivery of CpG oligonucleotide. DNA nanoribbons with different dimensions and patterns were assembled with long RCA strands and several short staples. Significantly, we demonstrated they exhibited high-efficiency cellular uptake and improved immunostimulatory activity compared with ss- or ds- DNA.
由于其合适的尺寸、明确的结构和低毒性，DNA纳米结构最近已成为一种药物递送纳米载体。在此，我们展示了一种利用滚环扩增（RCA）组装DNA纳米带结构并递送CpG寡核苷酸的方案。具有不同尺寸和图案的DNA纳米带通过长RCA链和几条短链组装而成。值得注意的是，我们证明与单链或双链DNA相比，它们表现出高效的细胞摄取和改善的免疫刺激活性。
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