Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, 243 122, Bareilly, Uttar Pradesh, India.
Int Immunopharmacol. 2013 Sep;17(1):65-70. doi: 10.1016/j.intimp.2013.05.019. Epub 2013 Jun 6.
We evaluated whether the nanoformulation of curcumin could be more effective than free curcumin against arsenic-induced immune dysfunction in rats. Curcumin was encapsulated in polylactic-co-glycolic acid (PLGA). Nanocurcumin (CUR-NP) exhibited a spherical shape with the mean particle size of 130.8 nm. Rats were randomly divided into five groups of six each. Group I was kept as the control. In Group II, rats were exposed to sodium arsenite (25 ppm) daily through drinking water for 42 days. Groups III, IV and V were treated with arsenic as in Group II, however, they were administered with nanoparticle, curcumin (100 mg/kg bw) and CUR-NP (100 mg/kg bw), respectively, by oral gavage during the last 14 days of arsenic exposure. At term, serum and spleen were collected. Immune dysfunction was evaluated by assessing cellular and humoral immunities. Arsenic significantly decreased the splenic lymphocyte proliferation in response to the antigen -- Keyhole Limpet Hemocyanin (KLH) and mitogen -- concanavalin-A. Arsenic reduced both the delayed type hypersensitivity response and secondary antibody (IgG) response to KLH. It also reduced the lipopolysaccharide-stimulated nitric oxide production in splenic lymphocytes. Free curcumin and CUR-NP treatment significantly attenuated these arsenic-mediated effects. However, the magnitude of the effects indicates that CUR-NP has better ameliorative potential than free curcumin at the equivalent dose level.
我们评估了姜黄素的纳米制剂是否比游离姜黄素更能有效对抗砷诱导的大鼠免疫功能障碍。姜黄素被包裹在聚乳酸-共-羟基乙酸（PLGA）中。纳米姜黄素（CUR-NP）呈球形，平均粒径为 130.8nm。大鼠随机分为五组，每组 6 只。第 I 组作为对照组。第 II 组大鼠通过饮用水每天摄入亚砷酸钠（25ppm），持续 42 天。第 III、IV 和 V 组以与第 II 组相同的方式暴露于砷，但在砷暴露的最后 14 天，通过口服灌胃分别用纳米粒子、姜黄素（100mg/kgbw）和 CUR-NP（100mg/kgbw）治疗。在期末，收集血清和脾脏。通过评估细胞和体液免疫来评估免疫功能障碍。砷显著降低了脾淋巴细胞对抗原--血蓝蛋白（KLH）和有丝分裂原--刀豆球蛋白 A 的增殖反应。砷降低了迟发型超敏反应和对 KLH 的二次抗体（IgG）反应。它还降低了脂多糖刺激的脾淋巴细胞中一氧化氮的产生。游离姜黄素和 CUR-NP 治疗显著减轻了这些砷介导的作用。然而，这些效应的幅度表明，在等效剂量水平下，CUR-NP 比游离姜黄素具有更好的改善潜力。
