Zakaria Hanine, El Kurdi Riham, Patra Digambara
Department of Chemistry, American University of Beirut Beirut Lebanon
RSC Adv. 2022 Oct 4;12(43):28245-28253. doi: 10.1039/d2ra01679f. eCollection 2022 Sep 28.
The main purpose of this paper is to design curcumin loaded PLGA nanocapsules for the selective detection of dopamine using fluorescence spectroscopy. In the present work curcumin loaded PLGA nanocapsules were synthesized using a solid-in-oil-in water (s/o/w) emulsion technique. The prepared nanocapsules were coated with a poly(diallyldimethylammonium)chloride (PDDA) polymer to increase the entrapment of curcumin into the core of PLGA polymer. PLGA-Cur-PDDA nanocapsules were characterized using different microscopic and spectroscopic techniques. Unlike free curcumin, the formed CUR-PLGA-PDDA NCs were established as nanoprobes for the selective detection of dopamine molecules. The selectivity and specificity of nanocapsules toward dopamine was achieved by measuring the fluorescence emission spectra of the NCs in the presence of other interference molecules such as tryptophan, melamine, adenine, It was noticed that increasing the concentration of the different molecules had no significant change in the fluorescence signal of the nanocapsules. These results confirm the strong quenching between dopamine and curcumin in the nanocapsules. Hence, this fluorescence emission technique was found to be selective, easy and fast with low cost for the determination of dopamine in a concentration range up to 5 mM with a detection limit equal to 22 nM.
本文的主要目的是设计负载姜黄素的聚乳酸-羟基乙酸共聚物（PLGA）纳米胶囊，用于利用荧光光谱法选择性检测多巴胺。在本研究中，采用水包油包固体（s/o/w）乳液技术合成了负载姜黄素的PLGA纳米胶囊。制备的纳米胶囊用聚二烯丙基二甲基氯化铵（PDDA）聚合物包被，以增加姜黄素在PLGA聚合物核中的包封率。使用不同的显微镜和光谱技术对PLGA-姜黄素-PDDA纳米胶囊进行了表征。与游离姜黄素不同，形成的CUR-PLGA-PDDA纳米胶囊被确立为用于选择性检测多巴胺分子的纳米探针。通过测量纳米胶囊在色氨酸、三聚氰胺、腺嘌呤等其他干扰分子存在下的荧光发射光谱，实现了纳米胶囊对多巴胺的选择性和特异性。结果发现，增加不同分子的浓度对纳米胶囊的荧光信号没有显著影响。这些结果证实了纳米胶囊中多巴胺与姜黄素之间存在强烈的猝灭作用。因此，该荧光发射技术被发现具有选择性、简便、快速且成本低的特点，可用于测定浓度高达5 mM的多巴胺，检测限为22 nM。
