Bogdanis G C, Stavrinou P, Fatouros I G, Philippou A, Chatzinikolaou A, Draganidis D, Ermidis G, Maridaki M
Department of Physical Education and Sport Science, University of Athens, Greece.
Food Chem Toxicol. 2013 Nov;61:171-7. doi: 10.1016/j.fct.2013.05.046. Epub 2013 Jun 6.
This study investigated the changes in oxidative stress biomarkers and antioxidant status indices caused by a 3-week high-intensity interval training (HIT) regimen. Eight physically active males performed three HIT sessions/week over 3 weeks. Each session included four to six 30-s bouts of high-intensity cycling separated by 4 min of recovery. Before training, acute exercise elevated protein carbonyls (PC), thiobarbituric acid reactive substances (TBARS), glutathione peroxidase (GPX) activity, total antioxidant capacity (TAC) and creatine kinase (CK), which peaked 24h post-exercise (252 ± 30%, 135 ± 17%, 10 ± 2%, 85 ± 14% and 36 ± 13%, above baseline, respectively; p<0.01), while catalase activity (CAT) peaked 30 min post-exercise (56 ± 18% above baseline; p<0.01). Training attenuated the exercise-induced increase in oxidative stress markers (PC by 13.3 ± 3.7%; TBARS by 7.2 ± 2.7%, p<0.01) and CK activity, despite the fact that total work done was 10.9 ± 3.6% greater in the post- compared with the pre-training exercise test. Training also induced a marked elevation of antioxidant status indices (TAC by 38.4 ± 7.2%; CAT by 26.2 ± 10.1%; GPX by 3.0 ± 0.6%, p<0.01). Short-term HIT attenuates oxidative stress and up-regulates antioxidant activity after only nine training sessions totaling 22 min of high intensity exercise, further supporting its positive effect not only on physical conditioning but also on health promotion.
本研究调查了为期3周的高强度间歇训练（HIT）方案所引起的氧化应激生物标志物和抗氧化状态指标的变化。八名身体活跃的男性在3周内每周进行三次HIT训练。每次训练包括四至六组30秒的高强度骑行，每组之间间隔4分钟的恢复时间。训练前，急性运动使蛋白质羰基（PC）、硫代巴比妥酸反应性物质（TBARS）、谷胱甘肽过氧化物酶（GPX）活性、总抗氧化能力（TAC）和肌酸激酶（CK）升高，这些指标在运动后24小时达到峰值（分别比基线高252±30%、135±17%、10±2%、85±14%和36±13%；p<0.01），而过氧化氢酶活性（CAT）在运动后30分钟达到峰值（比基线高56±18%；p<0.01）。尽管训练后与训练前运动测试相比，总运动量增加了10.9±3.6%，但训练减弱了运动诱导的氧化应激标志物（PC降低13.3±3.7%；TBARS降低7.2±2.7%，p<0.01）和CK活性的增加。训练还显著提高了抗氧化状态指标（TAC升高38.4±7.2%；CAT升高26.2±10.1%；GPX升高3.0±0.6%，p<0.01）。仅经过九次总计22分钟的高强度运动训练后，短期HIT即可减轻氧化应激并上调抗氧化活性，这进一步支持了其不仅对身体锻炼而且对健康促进的积极作用。
