College of Biological Science & Biotechnology, Beijing Forestry University, 100083 Beijing, China.
Food Chem Toxicol. 2013 Sep;59:67-71. doi: 10.1016/j.fct.2013.05.040. Epub 2013 Jun 5.
In order to investigate the binding ability of Lactobacillus strains to Benzo(a)pyrene (BaP), 15 strains were analysed. L. plantarum CICC 22135 and L. pentosus CICC 23163 exhibited high efficiency in removing BaP from aqueous medium; the binding rates were 66.76% and 64.31%, respectively. This process was affected by temperature, incubation time and pH, and cell viability was not necessary for the binding ability. Additionally, both strains, especially strain CICC 23163 showed high specificity in binding BaP. The cell-BaP complexes were stable in aqueous medium. The mechanism of binding was investigated by examining the binding ability of different components of the microorganism cells. The results revealed that peptidoglycans played an important role in binding BaP and its structural integrity was required. Consequently, we proposed that the mechanism of this process was a physisorption and peptidoglycan was the main binding site. These two strains may be used for dietary detoxification in human diet and animal feed.
为了研究乳酸菌对苯并(a)芘（BaP）的结合能力，对 15 株菌进行了分析。植物乳杆菌 CICC 22135 和戊糖片球菌 CICC 23163 对水相中 BaP 的去除效率较高，结合率分别为 66.76%和 64.31%。该过程受温度、孵育时间和 pH 值的影响，细胞活力对结合能力不是必需的。此外，这两种菌株，特别是 CICC 23163 菌株，对 BaP 具有高特异性结合能力。细胞-BaP 复合物在水介质中稳定。通过考察微生物细胞不同成分的结合能力，研究了结合机制。结果表明，肽聚糖在结合 BaP 中起重要作用，其结构完整性是必需的。因此，我们提出该过程的机制是一种物理吸附，肽聚糖是主要的结合位点。这两种菌株可能用于人类饮食和动物饲料中的膳食解毒。
