Xu Shu, Luo Jianying, Pan Xiayan, Liang Xiaoyu, Wu Jian, Zheng Wenjun, Chen Changjun, Hou Yiping, Ma Hongyu, Zhou Mingguo
Nanjing Agricultural University, Nanjing, China.
Biochim Biophys Acta. 2013 Aug;1834(8):1660-70. doi: 10.1016/j.bbapap.2013.05.023. Epub 2013 Jun 7.
The plant-pathogenic bacterium Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight, which is one of the most serious diseases of rice. Xoo has been studied for over one century, and much has been learned about it, but proteomic investigation has been neglected. In this study, proteome reference maps of Xoo were constructed by two-dimensional gel electrophoresis, and 628 spots in the gels representing 469 different protein species were identified with MALDI-TOF/TOF MS. The identified spots were assigned to 15 functional categories according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the annotations from the National Center for Biotechnology Information (NCBI) database. The data set has been deposited in the World-2DPAGE database (Database ID: 0044). In addition, comparative proteomic analysis revealed that proteins related to the TonB-dependent transportation system and energy metabolism are involved in the phenazine-1-carboxylic acid resistance in Xoo. In conclusion, we have established a proteome database for Xoo and have used this database in a comparative proteomic analysis that identified proteins potentially contributing to phenazine-1-carboxylic acid resistance in Xoo.
植物病原菌水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，简称Xoo）是水稻细菌性条斑病的病原体，该病是水稻最严重的病害之一。对Xoo的研究已有一个多世纪，人们对它已经有了很多了解，但蛋白质组学研究却被忽视了。在本研究中，通过二维凝胶电泳构建了Xoo的蛋白质组参考图谱，并用基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF MS）鉴定了凝胶中的628个斑点，这些斑点代表469种不同的蛋白质。根据京都基因与基因组百科全书（KEGG）数据库以及美国国立生物技术信息中心（NCBI）数据库的注释，将鉴定出的斑点分为15个功能类别。该数据集已存入世界二维电泳数据库（数据库ID：0044）。此外，比较蛋白质组学分析表明，与TonB依赖性转运系统和能量代谢相关的蛋白质参与了Xoo对吩嗪-1-羧酸的抗性。总之，我们建立了一个Xoo的蛋白质组数据库，并在比较蛋白质组学分析中使用了该数据库，该分析鉴定出了可能有助于Xoo对吩嗪-1-羧酸产生抗性的蛋白质。
