Programa de Pós-graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Brazil.
Exp Parasitol. 2013 Sep;135(1):24-9. doi: 10.1016/j.exppara.2013.05.014. Epub 2013 Jun 6.
Phytotherapy has been described as an alternative method for the control of gastrointestinal nematodes in small ruminants. Goal of the encapsulation of essential oils in biopolymer matrices is to optimize the biological effects of these oils. The aim of the present study was to evaluate the in vitro and in vivo anthelmintic activity of encapsulated Eucalyptus staigeriana essential oil (EncEs) on the eggs and larvae of Haemonchus contortus. Therefore, the egg hatching test (EHT), larval development test (LDT) and worm load evaluation were performed to evaluate Meriones unguiculatus experimentally infected with H. contortus. The chemical constituents of E. staigeriana essential oil (EsEO) and the in vitro oil release profile from the chitosan matrix at a pH of 1.2 and 7.0 were also characterized. EncEs and EsEO inhibited larval hatching by 97.19% and 99.96% at doses of 1.5 and 1.0 mg ml(-1), respectively. In the LDT, EncEs and EsEO induced a larvicidal effect greater than 95% at concentrations of 5.8 and 8 mg ml(-1), respectively. EncEs and EsEO decreased H. contortus load in M. unguiculatus by 40.51% and 46.44%, respectively. The major chemical constituents of EsEO were (+)-Limonene (72.9%), 1,8-Cineole (9.5%) and o-Cimene (4.6%). The release profile of EsEO was 30% in acid and 25% at neutral pH. The similar efficacy of EncEs and EsEO demonstrates that there was no optimization of anthelmintic action following the encapsulation process. Therefore, the use of new encapsulation matrices with controlled release in the pH of the abomasum should be investigated.
植物疗法被描述为控制小反刍动物胃肠道线虫的替代方法。将精油包封在生物聚合物基质中是为了优化这些油的生物学效应。本研究的目的是评估包封桉树 staigeriana 精油（EncEs）对捻转血矛线虫卵和幼虫的体外和体内驱虫活性。因此，进行了卵孵化试验（EHT）、幼虫发育试验（LDT）和蠕虫负荷评估，以评估实验感染捻转血矛线虫的毛尾沙鼠。还对桉树 staigeriana 精油（EsEO）的化学成分和壳聚糖基质在 pH 值为 1.2 和 7.0 时的体外油释放曲线进行了表征。EncEs 和 EsEO 在 1.5 和 1.0 mg ml(-1)的剂量下分别抑制幼虫孵化 97.19%和 99.96%。在 LDT 中，EncEs 和 EsEO 在 5.8 和 8 mg ml(-1)的浓度下诱导的杀幼虫作用大于 95%。EncEs 和 EsEO 分别使毛尾沙鼠中的捻转血矛线虫负荷减少 40.51%和 46.44%。EsEO 的主要化学成分是（+）-柠檬烯（72.9%）、1,8-桉叶素（9.5%）和 o-桧烯（4.6%）。EsEO 的释放曲线在酸性条件下为 30%，在中性 pH 值下为 25%。EncEs 和 EsEO 的功效相似表明，包封过程后驱虫作用没有得到优化。因此，应该研究在皱胃 pH 值下具有控释作用的新型包封基质的应用。
