Elbhnsawi Nagwa A, Elwakil Bassma H, Hassanin Ahmed H, Shehata Nader, Elshewemi Salma Sameh, Hagar Mohamed, Olama Zakia A
Department of Botany & Microbiology, Faculty of Science, Alexandria University, Alexandria 21568, Egypt.
Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria 21500, Egypt.
Membranes (Basel). 2023 Jun 15;13(6):604. doi: 10.3390/membranes13060604.
Accelerated wound healing in infected skin is still one of the areas where current therapeutic tactics fall short, which highlights the critical necessity for the exploration of new therapeutic approaches. The present study aimed to encapsulate oil in a nano-drug carrier to enhance its antimicrobial activity. Furthermore, in vitro, and in vivo wound healing studies of the novel nano-chitosan/ oil/cellulose acetate electrospun nanofibers were investigated. oil showed a potent antimicrobial activity against the tested pathogens and the highest inhibition zone diameter, MIC, and MBC (15.3 mm, 16.0 μg/mL, and 256 μg/mL, respectively) were recorded against . Data indicated a three-fold increase in the antimicrobial activity of oil encapsulated chitosan nanoparticle (43 mm inhibition zone diameter against ). The biosynthesized nanoparticles had a 48.26 nm particle size, 19.0 mV zeta potential, and 0.45 PDI. Electrospinning of nano-chitosan/ oil/cellulose acetate nanofibers was conducted, and the physico-chemical and biological properties revealed that the synthesized nanofibers were homogenous, with a thin diameter (98.0 nm) and a significantly high antimicrobial activity. The in vitro cytotoxic effect in a human normal melanocyte cell line (HFB4) proved an 80% cell viability using 1.5 mg/mL of nano-chitosan/ oil/cellulose acetate nanofibers. In vitro and in vivo wound healing studies revealed that nano-chitosan/ oil/cellulose acetate nanofibers were safe and efficiently enhanced the wound-healing process through enhancing TGF-β, type I and type III collagen production. As a conclusion, the manufactured nano-chitosan/ oil/cellulose acetate nanofiber showed effective potentiality for its use as a wound healing dressing.
在感染性皮肤中加速伤口愈合仍是当前治疗策略的不足之处，这凸显了探索新治疗方法的迫切必要性。本研究旨在将油封装在纳米药物载体中以增强其抗菌活性。此外，还对新型纳米壳聚糖/油/醋酸纤维素电纺纳米纤维进行了体外和体内伤口愈合研究。油对测试病原体显示出强大的抗菌活性，对其记录的最大抑菌圈直径、最低抑菌浓度和最低杀菌浓度分别为15.3毫米、16.0微克/毫升和256微克/毫升。数据表明，包封油的壳聚糖纳米颗粒的抗菌活性提高了三倍（对其抑菌圈直径为43毫米）。生物合成的纳米颗粒粒径为48.26纳米，zeta电位为19.0毫伏，多分散指数为0.45。进行了纳米壳聚糖/油/醋酸纤维素纳米纤维的电纺，其物理化学和生物学性质表明，合成的纳米纤维均匀，直径细（98.0纳米）且抗菌活性显著高。在人正常黑素细胞系（HFB4）中的体外细胞毒性作用证明，使用1.5毫克/毫升的纳米壳聚糖/油/醋酸纤维素纳米纤维时细胞活力为80%。体外和体内伤口愈合研究表明，纳米壳聚糖/油/醋酸纤维素纳米纤维安全且通过增强转化生长因子-β、I型和III型胶原蛋白的产生有效地促进了伤口愈合过程。总之，所制备的纳米壳聚糖/油/醋酸纤维素纳米纤维显示出作为伤口愈合敷料的有效潜力。
