School of Control and Computer Engineering, North China Electric Power University, Beijing 102206, China.
ACS Appl Mater Interfaces. 2013 Jun 26;5(12):5392-6. doi: 10.1021/am401493d. Epub 2013 Jun 12.
A mechanism is developed to construct a logic system by employing DNA/gold nanoparticle (AuNP) conjugates as a basic work unit, utilizing a fluorescent beacon probe to detect output signals. To implement the logic circuit, a self-assembly DNA structure is attached onto nanoparticles to form the fluorescent beacon. Moreover, assisted by regulation of multilevel strand displacement, cascaded logic gates are achieved. The computing results are detected by methods using fluorescent signals, gel electrophoresis and transmission electron microscope (TEM). This work is expected to demonstrate the feasibility of the cascaded logic system based on fluorescent nanoparticle beacons, suggesting applications in DNA computation and biotechnology.
我们开发了一种机制，通过将 DNA/金纳米粒子 (AuNP) 复合物用作基本工作单元，利用荧光示踪探针来检测输出信号，从而构建逻辑系统。为了实现逻辑电路，将自组装 DNA 结构附着在纳米粒子上以形成荧光示踪物。此外，通过多级链置换的调控，实现了级联逻辑门。通过荧光信号、凝胶电泳和透射电子显微镜 (TEM) 等方法检测计算结果。这项工作有望证明基于荧光纳米粒子示踪物的级联逻辑系统的可行性，这表明其在 DNA 计算和生物技术中有应用的潜力。
