Xing Ya-juan, Ji Jun-yuan, Zheng Ping, Zhang Ji-qiang, Ghulam Abbas
Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China.
Appl Microbiol Biotechnol. 2014 Feb;98(3):1357-66. doi: 10.1007/s00253-013-5003-x. Epub 2013 Jun 9.
The compartmentalized anaerobic reactor (CAR) is a patent novel high-rate reactor, which consists of three compartments. The reactor has a great potential for application due to its many advantages. In this work, the microbial consortium, spatial distribution, and their relationship with performance of CAR were investigated by means of polymerase chain reaction, denaturing gradient gel electrophoresis, and fluorescence in situ hybridization. The results showed that the predominant archaea were Methanobacterium, Methanosaeta, and Methanospirillum, and the predominant bacteria were Firmicutes, Deltaproteobacteria, Spirochaetes, Actinobacteria, and Gammaproteobacteria in the microbial consortium. The methanogenic archaea (MA), the hydrogen-producing acetogenic bacteria (HAB), and the hydrolytic fermentative bacteria (HFB) were found to be predominant in the upper, middle, and bottom compartments, respectively. The results revealed that the granular sludge took on a stratified microbial structure. The HFB, HAB, and MA were located in the outer shell, middle layer, and core, respectively. The microbial populations from the bottom compartment were relatively homogeneous in the granular sludge, and from the middle and upper compartments, they were relatively heterogeneous in the granular sludge. The microbial consortia and their spatial distribution were in accordance with the organic loading rate and chemical components in the three compartments.
分隔式厌氧反应器（CAR）是一种获得专利的新型高速率反应器，它由三个隔室组成。该反应器因其诸多优点而具有巨大的应用潜力。在这项工作中，通过聚合酶链反应、变性梯度凝胶电泳和荧光原位杂交等方法，研究了CAR的微生物群落、空间分布及其与性能的关系。结果表明，微生物群落中主要的古菌是甲烷杆菌属、甲烷八叠球菌属和甲烷螺菌属，主要的细菌是厚壁菌门、δ-变形菌纲、螺旋体门、放线菌门和γ-变形菌纲。发现产甲烷古菌（MA）、产氢产乙酸细菌（HAB）和水解发酵细菌（HFB）分别在上部、中部和底部隔室中占主导地位。结果表明，颗粒污泥呈现出分层的微生物结构。HFB、HAB和MA分别位于外壳、中间层和核心部位。底部隔室颗粒污泥中的微生物种群相对均匀，而中部和上部隔室颗粒污泥中的微生物种群相对不均匀。微生物群落及其空间分布与三个隔室中的有机负荷率和化学成分一致。
