Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4118-4123. doi: 10.1099/ijs.0.049841-0. Epub 2013 Jun 7.
A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated D1-W3(T), was isolated from tidal flat sediment of the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain D1-W3(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic analyses based on 16S rRNA gene sequences revealed that strain D1-W3(T) fell within the clade comprising species of the genus Loktanella, clustering with the type strains of Loktanella tamlensis, Loktanella rosea and Loktanella maricola, with which it exhibited the highest 16S rRNA gene sequence similarity values (98.1-98.2 %). The 16S rRNA gene sequence similarity values between strain D1-W3(T) and the type strains of other species of the genus Loktanella were in the range 93.5-96.5 %. The DNA G+C content of strain D1-W3(T) was 58.1 mol% and the mean DNA-DNA hybridization values with L. tamlensis KCTC 12722(T), L. rosea LMG 22534(T) and L. maricola DSW-18(T) were 13-25 %. Strain D1-W3(T) contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the predominant fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, showed that strain D1-W3(T) could be differentiated from other species of the genus Loktanella. On the basis of the data presented, strain D1-W3(T) represents a novel species of the genus Loktanella, for which the name Loktanella sediminilitoris sp. nov. is proposed. The type strain is D1-W3(T) ( = KCTC 32383(T) = CECT 8284(T)).
一株革兰氏阴性、非运动、杆状细菌菌株，命名为 D1-W3(T)，从韩国南海潮间带沉积物中分离得到，并采用多相分类法进行了分类学研究。菌株 D1-W3(T) 在 pH7.0-8.0、25°C 和 2%（w/v）NaCl 存在下生长最佳。基于 16S rRNA 基因序列的系统发育分析显示，菌株 D1-W3(T) 属于 Loktanella 属的一个分支，与 Loktanella tamlensis、Loktanella rosea 和 Loktanella maricola 的模式菌株聚类，与它们的 16S rRNA 基因序列相似度最高（98.1-98.2%）。菌株 D1-W3(T) 与 Loktanella 属其他种的模式菌株的 16S rRNA 基因序列相似度在 93.5-96.5%之间。菌株 D1-W3(T) 的 DNA G+C 含量为 58.1 mol%，与 Loktanella tamlensis KCTC 12722(T)、Loktanella rosea LMG 22534(T) 和 Loktanella maricola DSW-18(T) 的 DNA-DNA 杂交值为 13-25%。菌株 D1-W3(T) 含有 Q-10 作为主要的泛醌和 C18 : 1ω7c 作为主要的脂肪酸。主要的极性脂是磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的胺脂。表型特征的差异，以及系统发育和遗传上的独特性，表明菌株 D1-W3(T) 可以与 Loktanella 属的其他种区分开来。基于所提供的数据，菌株 D1-W3(T) 代表 Loktanella 属的一个新种，建议命名为 Loktanella sediminilitoris sp. nov.。模式菌株为 D1-W3(T)（=KCTC 32383(T)=CECT 8284(T)）。
