Zhang X, Wang Z, Kang Y, Li X, Ma X, Ma L
Department of Respiratory and Critical Care Medicine, People's Hospital of Zhengzhou University, Weiwu Road 7, Zhengzhou, Henan, China.
Clin Transl Oncol. 2014 Feb;16(2):178-83. doi: 10.1007/s12094-013-1057-6. Epub 2013 Jun 8.
MCAM has been recently identified as a biomarker for epithelial-mesenchymal transition (EMT) and is potentially involved in metastasis of cancer. The current study aimed at investigating the expression of MCAM in non-small-cell lung cancer (NSCLC) and its clinico-pathological significance.
A follow-up analysis was performed on 118 patients with NSCLC resected by lobectomy or pneumectomy with systematic lymph node dissection. All patients were followed for 6-60 months. Immunostaining of tissue sections from primary tumors and their lymph node metastasis was performed and evaluated using monoclonal antibody against MCAM, E-cadherin, and vimentin. Correlations were investigated between MCAM immunostaining in primary tumors and E-cadherin, vimentin immunostaining, lymph node metastasis, and survival.
MCAM protein expression was found in 46.61 % of squamous cell carcinomas and 37.47 % of adenocarcinomas; MCAM expression positively correlated with vimentin, but inversely with E-cadherin (both P values <0.05). There were significant correlations between the MCAM immunostaining score in primary tumors and in their lymph node metastasis (P = 0.03). According to the Kaplan-Meier survival estimate, the level of MCAM expression in primary tumors was a statistically significant prognostic factor (P < 0.05).
MCAM expression in surgically treated NSCLC is clearly associated with lymph node metastasis and poor prognosis.
MCAM最近被确定为上皮-间质转化（EMT）的生物标志物，并可能参与癌症转移。本研究旨在调查MCAM在非小细胞肺癌（NSCLC）中的表达及其临床病理意义。
对118例行肺叶切除术或全肺切除术并系统性淋巴结清扫的NSCLC患者进行随访分析。所有患者随访6-60个月。使用抗MCAM、E-钙黏蛋白和波形蛋白的单克隆抗体对原发性肿瘤及其淋巴结转移的组织切片进行免疫染色并评估。研究原发性肿瘤中MCAM免疫染色与E-钙黏蛋白、波形蛋白免疫染色、淋巴结转移和生存率之间的相关性。
在46.61%的鳞状细胞癌和37.47%的腺癌中发现MCAM蛋白表达；MCAM表达与波形蛋白呈正相关，但与E-钙黏蛋白呈负相关（P值均<0.05）。原发性肿瘤及其淋巴结转移中的MCAM免疫染色评分之间存在显著相关性（P = 0.03）。根据Kaplan-Meier生存估计，原发性肿瘤中MCAM的表达水平是一个具有统计学意义的预后因素（P < 0.05）。
手术治疗的NSCLC中MCAM的表达与淋巴结转移和预后不良明显相关。
